JCRB1997 UCCA-25

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1997 Cell Name   UCCA-25
Profile   Derived from colorectal cancer with ulcerative colitis. Isolated as adenosquamous carcinoma of the adenomas by cloning. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   33-year-old
Identity   available Tissue for Primary Cancer   Rectum moderately adenocarcinoma
Case history   Duration of UC 11years Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   DT=40.5 hrs
Classify   tumor Established by   Kazuya YAMASHITA
Registered by   YAMASHITA Kazuya Regulation for Distribution   
Comment    Year   
Medium   MEM+10%FBS Methods for Passages   0.25%Trypsin+0.02%EDTA, passage 1:20split /1 week
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   primary rectum carcinoma
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Reference
Pubmed id:16206288Disruption of the p53-p53r2 DNA repair system in ulcerative colitis contributes to colon tumorigenesis.
Yoshida T,Haga S,Numata Y,Yamashita K,Mikami T,Ogawa T,Ohkusa T,Okayasu I
Int J Cancer. 2006 Mar 15;118(6):1395-403
Pubmed id:15016319Unique characteristics of rectal carcinoma cell lines derived from invasive carcinomas in ulcerative colitis patients.
Yamashita K,Yasuda S,Kuba T,Otani Y,Fujiwara M,Okayasu I
Cancer Sci. 2004 Mar;95(3):211-7
Pubmed id:none細胞株を用いた免疫組織化学染色マルチプルコントロール試料の作製と標準化の基礎的検討.
山下和也, 他.
神奈川県臨床検査技師会雑誌(2188-6628)

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LOT Information

Cell No.   JCRB1997 Cell Name   UCCA-25
LOT No.   01242024 Lot Specification   distribution
Medium   MEM (GIBCO cat.No.11095-080) with 10% fetal bovine serum (not HI, SIGMA, Cat#172012, Lot#12J396) Temperature   37 C
Cell Density at Seeding   1.0-2.0x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin (GIBCO) and 0.02% EDTA. (Split ratio=1/20 /week)
Doubling Time   NT Cell Number in Vial (cells/1ml)   5.0x10^6
Viability at cell freezing (%)   99.00 Antibiotics Used   free
Passage Number   p6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12
D13S317:8
D7S820:11,12
D16S539:10
VWA:15
TH01:9
AM:X
TPOX:8
CSF1PO:10,11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   87 Additional information   We recommend to start culture with 25T flask,cause of low adhesion rate.
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