JCRB9005 BALB/3T3 clone A31

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9005 Cell Name   BALB/3T3 clone A31
Profile   fibroblast, contact inhibited, (BALB/c mouse) Other Name   BALB/3T3
Animal   mouse Strain   BALB/c
Genus   Mus Species   musclus
Sex    Age   
Identity    Tissue for Primary Cancer   embryo
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span    Crisis PDL   
Morphology    Character   contact inhibited
Classify    Established by   Aaronson & Todaro
Registered by   Mizusawa,H. Regulation for Distribution   
Comment   Prepared from CCL163. Year   1985
Medium   Dulbecco's modified Eagle's medium with 10% calf serum Methods for Passages   0.02% EDTA & 0.25% trypsin. Subculture every 3 days. Never allow cultures to become completely confluent.
Cell Number on Passage   3x10^5 cells/60mm dish Race   
CO2 Conc.   5 % Tissue Sampling   embryo
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0184
Reference
Pubmed id:7394516Cell variants showing differential susceptibility to ultraviolet light--induced transformation.
Kakunaga T,Crow JD
Science. 1980 Jul 25;209(4455):505-7
Pubmed id:4311790Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells.
Jainchill JL,Aaronson SA,Todaro GJ
J Virol. 1969 Nov;4(5):549-53
Pubmed id:4301006Development of 3T3-like lines from Balb-c mouse embryo cultures: transformation susceptibility to SV40.
Aaronson SA,Todaro GJ
J Cell Physiol. 1968 Oct;72(2):141-8
Pubmed id:13985244Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines.
TODARO GJ,GREEN H
J Cell Biol. 1963 May;17():299-313

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LOT Information

Cell No.   JCRB9005 Cell Name   
LOT No.   11202009 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx. 2 - 3 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   2.1 x 10^6
Viability at cell freezing (%)   93.7 Antibiotics Used   free
Passage Number   P72 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9005 Cell Name   
LOT No.   9005 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% calf serum Temperature   37C
Cell Density at Seeding   1 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in PBS
Doubling Time   16hr Cell Number in Vial (cells/1ml)   1.2x 10^5
Viability at cell freezing (%)   90 Antibiotics Used   free
Passage Number   P72 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   70% Culture medium + 20% FCS + 10% DMSO CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9005 Cell Name   
LOT No.   12102007 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% calf serum Temperature   37C
Cell Density at Seeding   1x10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in PBS
Doubling Time   16hr Cell Number in Vial (cells/1ml)   6.0 x 10^5
Viability at cell freezing (%)   91 Antibiotics Used   free
Passage Number   P72 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   70% Culture medium + 20% CS + 10% DMSO CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9005 Cell Name   
LOT No.   08072003 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% calf serum Temperature   37 C
Cell Density at Seeding   2 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   D.T. = ca. 24 hrs Cell Number in Vial (cells/1ml)   7.3 x 10^5
Viability at cell freezing (%)   92.7 Antibiotics Used   free
Passage Number   P72 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% CS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9005 Cell Name   BALB/3T3 clone A31
LOT No.   111695 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's minimal medium with 10% calf serum (ICN Flow 1059023). Temperature   37 C
Cell Density at Seeding   8.4x10^4 cells/ml Methods for Passages   Wash cells with PBS(-) and treated with 0.25%-trypsin and 0.02% EDTA for 10min. at 37 C.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.9x10^6
Viability at cell freezing (%)   97.0 Antibiotics Used   free
Passage Number   P70 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB9005 Cell Name   BALB/3T3 clone A31
LOT No.   F00963 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum (BioWhittaker, 3MC0581) Temperature   37 C
Cell Density at Seeding   2.5-3.2x10^4 cells/ml Methods for Passages   Cells were treated with 0.02% EDTA and 0.25% trypsin (Difco 1:250)
Doubling Time   NT Cell Number in Vial (cells/1ml)   7.2x10^5
Viability at cell freezing (%)   77.2 Antibiotics Used   free
Passage Number   P69 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9005 Cell Name   BALB/3T3 clone A31
LOT No.   090993 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% calf serum (Hyclone, 2111480) Temperature   37 C
Cell Density at Seeding   7-8x10^4 cells/ml Methods for Passages   Wash cells with PBS(-) and treated with 0.25% trypsin and 0.02% EDTA for 5-15min. at 37 C.
Doubling Time   15.5 hours. Cell Number in Vial (cells/1ml)   1.5x10^6
Viability at cell freezing (%)   96.5 Antibiotics Used   free
Passage Number   P70 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   NT Isozyme Analysis   G6PD, LD, Pep B
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9005 Cell Name   BALB/3T3 clone A31
LOT No.   082493 Lot Specification   distribution
Medium   Cells discarded. Temperature   
Cell Density at Seeding    Methods for Passages   
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   G6PD, LD, PepB
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9005 Cell Name   BALB/3T3 clone A31
LOT No.   101691 Lot Specification   distribution
Medium   DMEM + CS 10% Temperature   37 C
Cell Density at Seeding   3x10^5/6D Methods for Passages   0.02% EDTA and 0.25% trypsin. Subculture every 3 days. Never allow cultures to become completely confluent.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.0x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P71 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9005 Cell Name   BALB/3T3 clone A31
LOT No.   100890 Lot Specification   distribution
Medium   DMEM + CS 10% Temperature   37
Cell Density at Seeding   3x10^5/6D Methods for Passages   0.02% EDTA and 0.25% trypsin. Subculture every 3 days. Never allow cultures to become completely confluent.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.5x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P71 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9005 Cell Name   BALB/3T3 clone A31
LOT No.   090888 Lot Specification   distribution
Medium   DMEM + CS 10% Temperature   37
Cell Density at Seeding   3x10^5/6D Methods for Passages   0.02% EDTA and 0.25% trypsin. Subculture every 3 days. Never allow cultures to become completely confluent.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.1x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P70 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9005 Cell Name   BALB/3T3 clone A31
LOT No.   03212013 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099). The vial is incorrectly labeled as 03262013. Temperature   37 C
Cell Density at Seeding   1 - 2.4 x 10^4 cells / mL Methods for Passages   0.02% EDTA & 0.25% trypsin. Subculture every 3 days. Never allow cultures to become completely confluent.
Doubling Time    Cell Number in Vial (cells/1ml)   7.1 x 10^5
Viability at cell freezing (%)   98.6 Antibiotics Used   free
Passage Number   p72 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   10% DMSO, 20% FBS, DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   98.6 Additional information   
Cell No.   JCRB9005 Cell Name   BALB/3T3 clone A31
LOT No.   10062016 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   approx. 3.5 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Never allow cultures to become completely confluent.
Doubling Time   approx. 28 hrs Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   97 Antibiotics Used   free
Passage Number   P72 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   97 Additional information   
Cell No.   JCRB9005 Cell Name   BALB/3T3 clone A31
LOT No.   08062021 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; BioWest Cat. # S1820-500) Temperature   37 C
Cell Density at Seeding   4.2 - 8.2 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 31 hrs. Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   99.2 Antibiotics Used   free
Passage Number   P72 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   5% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   96.0 Additional information   
Cell No.   JCRB9005 Cell Name   BALB/3T3 clone A31
LOT No.   09052024 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (GIBCO:catalog No.11885-084) with 10% fetal bovine serum (Nichirei Cat. # 174112, Lot 20M00K). Temperature   37 C
Cell Density at Seeding   1-1.4x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.5x10^6
Viability at cell freezing (%)   99 Antibiotics Used   free
Passage Number   p72 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   96.6 Additional information   
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