JCRB9046 GH1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9046 Cell Name   GH1
Profile   pituitary tumor, somatotrophin-secreting, (Wistar/Furth rat) Other Name   
Animal   rat Strain   Wistar/Furth
Genus   Rattus Species   norvegicus
Sex    Age   
Identity    Tissue for Primary Cancer   pituitary
Case history    Metastasis   
Tissue Metastasized    Genetics   Pituitary tumor
Life Span    Crisis PDL   
Morphology    Character   Pituitary tumor, somatotrophin secreting
Classify    Established by   Yasumura,Y. et al.
Registered by    Regulation for Distribution   Following rules from ATCC
Comment   Prepared from CCL 82. Year   1986
Medium   Ham's F10 medium with 15% horse serum and 2.5% fetal calf serum. Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin
Cell Number on Passage   split ratio=1/2 - 1/3 Race   
CO2 Conc.   5 % Tissue Sampling   pituitary gland
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0610
Reference
Pubmed id:4951281Establishment of clonal strains of rat pituitary tumor cells that secrete growth hormone.
Tashjian AH Jr,Yasumura Y,Levine L,Sato GH,Parker ML
Endocrinology. 1968 Feb;82(2):342-52




LOT Information

Cell No.   JCRB9046 Cell Name   GH1
LOT No.   101292 Lot Specification   distribution
Medium   Ham F10(GIBCO 61D1512,081992) with 2.5% fetal bovine serum(Flow0070893)& 15% horse serum(JRS OD1223) Temperature   37 C
Cell Density at Seeding   1.8x10^5 cells/ml Methods for Passages   Treat cells with 0.25% trypsin and 0.02% EDTA. Cells stick hard and island like formation observed.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.2x10^6
Viability at cell freezing (%)   94.0 Antibiotics Used   free
Passage Number   P48 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9046 Cell Name   GH1
LOT No.   013087 Lot Specification   distribution
Medium   Ham's F10, 82.5%; horse serum, 15%; FCS, 2.5% Temperature   37
Cell Density at Seeding   1/2 - 1/3 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.4x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P49 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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