JCRB9050 IMR-32

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9050 Cell Name   IMR-32
Profile   Human neuroblastoma cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   13-month-old
Identity   available Tissue for Primary Cancer   neural
Case history   neuroblastoma arising at abdomen Metastasis   
Tissue Metastasized    Genetics   N-myc amplification
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   transplantable to nude mice
Classify   tumor Established by   Nichols, W.W. et al.
Registered by    Regulation for Distribution   
Comment   Prepared from CCL127. Year   1986
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% fetal calf serum. Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin
Cell Number on Passage   split ratio=1/3 - 1/6 Race   Caucasian
CO2 Conc.   5 % Tissue Sampling   neuroblastoma arising at abdomen
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0346
Reference
Pubmed id:2121721Monoclonal antibodies to disialogangliosides: characterization of antibody-mediated cytotoxicity against human melanoma and neuroblastoma cells in vitro.
Kawashima I,Tada N,Fujimori T,Tai T
J Biochem. 1990 Jul;108(1):109-15
Pubmed id:841332Enzyme polymorphisms as genetic signatures in human cell cultures.
O'Brien SU,Kleiner G,Olson R,Shannon JE
Science. 1977 Mar 25;195(4284):1345-8
Pubmed id:5459762Definition of a continuous human cell line derived from neuroblastoma.
Tumilowicz JJ,Nichols WW,Cholon JJ,Greene AE
Cancer Res. 1970 Aug;30(8):2110-8

Images



LOT Information

Cell No.   JCRB9050 Cell Name   
LOT No.   06092005 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   1 - 3 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Alternatively the cells can be harvested by pipetting only since the attachment is very loose.
Doubling Time   40 hrs Cell Number in Vial (cells/1ml)   4.5 x 10^6
Viability at cell freezing (%)   82 Antibiotics Used   free
Passage Number   P57 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9050 Cell Name   IMR-32
LOT No.   102594 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% heat inactivated fetal bovine serum & non-essential AAs) Temperature   37 C
Cell Density at Seeding   1.5-1.8x10^5 cells/sq.cm. Methods for Passages   Cells were washed with PBS(-) & treated with 0.25% trypsin and 0.02% EDTA at 37 C for 5-13 min.
Doubling Time   NT Cell Number in Vial (cells/1ml)   7.1x10^6
Viability at cell freezing (%)   88.8 Antibiotics Used   free
Passage Number   P58 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:9
D7S820:9,10
D16S539:8
VWA:15
TH01:7,10
AM:XY
TPOX:11
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB9050 Cell Name   IMR-32
LOT No.   120492 Lot Specification   distribution
Medium   Eagle`s MEM(Earle's BSS) with non-essential amino acids(Gibco 100x) with 10% FBS(Flow,0070893) Temperature   37 C
Cell Density at Seeding   >1.7x10^5cells/cm^2 Methods for Passages   Cells are treated with 0.05% trypsin and 0.02% EDTA. Cell attachment to culture vessel is loose.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.8x10^6
Viability at cell freezing (%)   87.0 Antibiotics Used   free
Passage Number   P61 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9050 Cell Name   IMR-32
LOT No.   030492 Lot Specification   distribution
Medium   MEM + NEAA + FBS 10% Temperature   37
Cell Density at Seeding   1.1x10^6 Methods for Passages   0.02 % EDTA + 0.2 % trypsin
Doubling Time   NT Cell Number in Vial (cells/1ml)   6.7x10^5
Viability at cell freezing (%)   69.3 Antibiotics Used   free
Passage Number   P56 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP,LD,G6PD
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion    Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9050 Cell Name   IMR-32
LOT No.   121791 Lot Specification   distribution
Medium   MEM + NEAA + FBS 10% Temperature   37
Cell Density at Seeding    Methods for Passages   0.02 % EDTA + 0.25 % trypsin
Doubling Time   NT Cell Number in Vial (cells/1ml)   6.62x10^5
Viability at cell freezing (%)   67.4 Antibiotics Used   free
Passage Number   P57 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9050 Cell Name   IMR-32
LOT No.   101890 Lot Specification   distribution
Medium   MEM w/ non essential amino acids + FCS 10% Temperature   37
Cell Density at Seeding   1/3 - 1/6 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.8x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P56 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9050 Cell Name   IMR-32
LOT No.   010490 Lot Specification   distribution
Medium   MEM w/ non essential amino acids + FCS 10% Temperature   37
Cell Density at Seeding   1/3 - 1/6 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.1x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P56 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9050 Cell Name   IMR-32
LOT No.   110288 Lot Specification   distribution
Medium   MEM w/ non essential amino acids + FCS 10% Temperature   37
Cell Density at Seeding   1/3 - 1/6 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   7.8x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P57 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LD,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9050 Cell Name   IMR-32
LOT No.   033087 Lot Specification   distribution
Medium   MEM w/ non essential amino acids + FCS 10% Temperature   37
Cell Density at Seeding   1/3 - 1/6 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.7x10^6
Viability at cell freezing (%)   92% Antibiotics Used   free
Passage Number   P55 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9050 Cell Name   
LOT No.   040997 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essent- ial amino acids and 10% fetal calf serum Temperature   37 C
Cell Density at Seeding   1-2 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   2.0 x 10^6
Viability at cell freezing (%)   84.0 Antibiotics Used   free
Passage Number   P57 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9050 Cell Name   IMR-32
LOT No.   07222016 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   0.9 - 1.2 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 39 hrs. Cell Number in Vial (cells/1ml)   5.7 x 10^6
Viability at cell freezing (%)   87 Antibiotics Used   free
Passage Number   P56 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM/NEAA CO2 Conc.   5%
Viability immediately after thawing (%)   87 Additional information   
Cell No.   JCRB9050 Cell Name   IMR-32
LOT No.   04032020 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   0.5 - 1.8 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Cell attachment is weak, and the cells may detach by PBS wash.
Doubling Time   approx. 36 hrs. Cell Number in Vial (cells/1ml)   3.4 x 10^6
Viability at cell freezing (%)   90 Antibiotics Used   free
Passage Number   P58 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   87 Additional information   
Cell No.   JCRB9050 Cell Name   IMR-32
LOT No.   06142024 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum (FBS; Nichirei Cat. # 174112 Lot 20M00K) Temperature   37 C
Cell Density at Seeding   8 - 9 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Cell attachment is weak, and the cells may detach by PBS wash.
Doubling Time   39 - 53 hrs. Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability at cell freezing (%)   77.9 Antibiotics Used   free
Passage Number   P57 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM with NEAA CO2 Conc.   5%
Viability immediately after thawing (%)   77.6 Additional information   
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