JCRB9075 NCTC Clone 1469

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9075 Cell Name   NCTC Clone 1469
Profile   Normal cell line from mouse liver. Other Name   NCTC Clone 1469
Animal   mouse Strain   C3H/An
Genus   Mus Species   musclus
Sex   M Age   
Identity    Tissue for Primary Cancer   liver
Case history    Metastasis   
Tissue Metastasized    Genetics   liver
Life Span    Crisis PDL   
Morphology   epithelial-like Character   liver
Classify   normal Established by   Hobbs, G.L., et al.
Registered by   Purchased from ATCC Regulation for Distribution   Following rules from ATCC
Comment   CCL 9.1. Year   1985
Medium   NCTC 135 medum with horse serum 10% Methods for Passages   Scraping.
Cell Number on Passage   1/2 - 1/4 Race   
CO2 Conc.   5 % Tissue Sampling   liver
Tissue Type   normal

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3066
Reference
Pubmed id:13766009A hepatitis virus complicating studies with mouse leukemia.
MANAKER RA,PICZAK CV,MILLER AA,STANTON MF
J Natl Cancer Inst. 1961 Jul;27():29-51
Pubmed id:14431674Glycogen content of cell suspensions prepared from massive tissue culture. II. Comparison of fourteen cell strains after long cultivation.
PEPPERS EV,WESTFALL BB,EARLE WR
J Natl Cancer Inst. 1959 Oct;23():823-31
Pubmed id:13619759Long-term cultivation of animal tissue cells in large cultures.
EARLE WR
Fed Proc. 1958 Dec;17(4):967-74
Pubmed id:13523589Studies on culture lines derived from mouse liver parenchymatous cells grown in long-term tissue culture.
EVANS VJ,HAWKINS NM,WESTFALL BB,EARLE WR
Cancer Res. 1958 Apr;18(3):261-6
Pubmed id:13502690Establishment of a clone of mouse liver cells from a single isolated cell.
HOBBS GL,SANFORD KK,EVANS VJ,EARLE WR
J Natl Cancer Inst. 1957 May;18(5):701-7
Pubmed id:14939026The growth in vitro of massive cultures of liver cells.
EVANS VJ,EARLE WR,WILSON EP,WALTZ HK,MACKEY CJ
J Natl Cancer Inst. 1952 Jun;12(6):1245-65

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LOT Information

Cell No.   JCRB9075 Cell Name   NCTC Clone 1469
LOT No.   081092 Lot Specification   distribution
Medium   NCTC135(Flow Laboratory lot#2010222) + horse serum 10% (J.R.Scientific lot#OD1223) Temperature   37C
Cell Density at Seeding   1x10^6 Methods for Passages   scraping (floating cells were also cultivated at the same time.)
Doubling Time   12Hrs. Cell Number in Vial (cells/1ml)   2.16x10^6
Viability at cell freezing (%)   97% Antibiotics Used   free
Passage Number   P21 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9075 Cell Name   
LOT No.   022398 Lot Specification   distribution
Medium   NCTC 135 medium with 10% horse serum Temperature   37 C
Cell Density at Seeding   1 x 10^5 cells/ml Methods for Passages   Scraping, or 0.25% trypsin and 0.02% EDTA
Doubling Time   ca. 48 hrs Cell Number in Vial (cells/1ml)   4.2 x 10^6
Viability at cell freezing (%)   92.0 Antibiotics Used   free
Passage Number   P19 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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