JCRB9076 FHs 74 Int

Cell information

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Characteristics of JCRB9076 FHs 74 Int

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9076 Cell Name   FHs 74 Int
Profile   Human fetal small intestin cells. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   intestine
Case history    Metastasis   
Tissue Metastasized    Genetics   fetal small intestine, normal
Life Span    Crisis PDL   
Morphology    Character   fetal small intestine, normal
Classify   normal Established by   Owens,R.
Registered by    Regulation for Distribution   
Comment   Prepared from CCL241. Culture medium w/ EGF 30ng/ml = doubling time 65 hr, w/o EGF = 168 hr. Cells of passage 20 can undergo 15 additional population doublings. Year   1986
Medium   DMEM(glucose 4.5g/l)+Insulin 10micro g/ml+FCS 10% Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin
Cell Number on Passage   1/2 Race   
CO2 Conc.   5 % Tissue Sampling   small intestine
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2899
Reference
Pubmed id:283258In vitro properties of epithelial cell lines established from human carcinomas and nonmalignant tissue.
Smith HS
J Natl Cancer Inst. 1979 Feb;62(2):225-30

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LOT Information

Cell No.   JCRB9076 Cell Name   
LOT No.   10112007 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (high glucose, 4.5 g/L) with 10 ug/ml insulin and 10% fetal bovine serum (FBS lor; GIBCO 484375) Temperature   37 C
Cell Density at Seeding   3 -9 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 4 days Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   95.3 Antibiotics Used   free
Passage Number   P27 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9076 Cell Name   
LOT No.   05272002 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (high glucose) with 10 ug/ml insulin and 10% fetal bovine serum Temperature   37 C
Cell Density at Seeding   2-8 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS
Doubling Time   D.T. = 70-80 hrs Cell Number in Vial (cells/1ml)   4.2 x 10^5
Viability at cell freezing (%)   95 Antibiotics Used   free
Passage Number   P27 PDL   12 at bank
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS-DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9076 Cell Name   FHs 74 Int
LOT No.   072188 Lot Specification   distribution
Medium   DMEM(glucose 4.5g/l)+Insulin 10micro g/ml+FCS 10% Temperature   37
Cell Density at Seeding   1/2 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   5.2x10^5
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P26 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,MPI,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9076 Cell Name   
LOT No.   081898 Lot Specification   distribution
Medium   Dulbecco's MEM (4.5 g/L glucose) with 10 ug/ml insulin and 10% fetal calf serum Temperature   37 C
Cell Density at Seeding   3-5 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Grow very slowly. Split ratio 1:2-1:3 is recommended.
Doubling Time   D.T.=ca 130 hrs Cell Number in Vial (cells/1ml)   6.1 x 10^5
Viability at cell freezing (%)   92.0 Antibiotics Used   free
Passage Number   P26 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9076 Cell Name   FHs 74 Int
LOT No.   06112020 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (high glucose) with 10 ug/mL insulin and 10% fetal bovien serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.3 - 3.8 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 88 hrs. Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   84.6 Antibiotics Used   free
Passage Number   P26 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM (high glucose) CO2 Conc.   5%
Viability immediately after thawing (%)   94.8 Additional information   
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