-Detailed Information [JCRB9085]-

JCRB9085 P3X63Ag8U.1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	JCRB9085	Cell Name	P3X63Ag8U.1
Profile	myeloma, plasmacytoma, 8-azaguanine resistant, (BALB/c mouse)	Other Name
Animal	mouse	Strain	BALB/C
Genus	Mus	Species	musclus
Sex		Age
Identity		Tissue for Primary Cancer	hemo-lymphocytic
Case history		Metastasis
Tissue Metastasized		Genetics	myeloma derivative
Life Span		Crisis PDL
Morphology		Character	myeloma derivative
Classify		Established by	Kohler & Milstein
Registered by		Regulation for Distribution	Following rules from ATCC
Comment	Prepared from CRL1597.	Year	1985
Medium	DMEM + 4.5g/L glucose + NEAA + FCS 10%	Methods for Passages	Dilution. A density of 1-1.5x10^6/ml should not be exceeded.
Cell Number on Passage	3-5 x 10^5/ml	Race
CO2 Conc.	5 %	Tissue Sampling	plasmacytoma
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	not tested				Detected RNA Virus	not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_3412

Reference
Pubmed id:3500068	Reciprocal effects of epidermal growth factor and transforming growth factor beta on the anchorage-dependent and -independent growth of A431 epidermoid carcinoma cells. Lee K,Tanaka M,Hatanaka M,Kuze F Exp Cell Res. 1987 Nov;173(1):156-62
Pubmed id:113458	A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines. Kearney JF,Radbruch A,Liesegang B,Rajewsky K J Immunol. 1979 Oct;123(4):1548-50
Pubmed id:74805	Hybrid cell lines secreting monoclonal antibody specific for major histocompatibility antigens of the mouse. Lemke H,Hammerling GJ,Hohmann C,Rajewsky K Nature. 1978 Jan 19;271(5642):249-51
Pubmed id:567551	Fusion of mouse myeloma and spleen cells. Yelton DE,Diamond BA,Kwan SP,Scharff MD Curr Top Microbiol Immunol. 1978;81():1-7
Pubmed id:268647	Production of antibodies against influenza virus by somatic cell hybrids between mouse myeloma and primed spleen cells. Koprowski H,Gerhard W,Croce CM Proc Natl Acad Sci U S A. 1977 Jul;74(7):2985-8
Pubmed id:1172191	Continuous cultures of fused cells secreting antibody of predefined specificity. Köhler G,Milstein C Nature. 1975 Aug 7;256(5517):495-7

LOT Information

03142002
111688

Cell No.	JCRB9085	Cell Name
LOT No.	03142002	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium (4.5 g/L glucose), non-essential amino acids, and 10% fetal bovine serum	Temperature	37 C
Cell Density at Seeding	1-2 x 10^5 cells/ml	Methods for Passages	Dilution
Doubling Time	D.T. = ca 18 hrs	Cell Number in Vial (cells/1ml)	3.0 x 10^6
Viability at cell freezing (%)	82	Antibiotics Used	free
Passage Number	Unknown (5 at cellbank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	No	Exoteric Gene
Medium for Freezing	culture medium with 10% DMSO, 20% FBS	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB9085	Cell Name	P3X63Ag8U.1
LOT No.	111688	Lot Specification	distribution
Medium	DMEM,4.5g/L glucose,non essen amino acids + FCS10%	Temperature	37
Cell Density at Seeding	3-5 x 10^5/ml	Methods for Passages	Dilution. A density of 1-1.5x10^6/ml should not be exceeded.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	9.7x10^6
Viability at cell freezing (%)	NT	Antibiotics Used	free
Passage Number	*P4	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	LD
Chromosome Mode		Chromosome Information	NT
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information