JCRB9091 Y3-Ag 1.2.3
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB9091 | Cell Name | Y3-Ag 1.2.3 |
|---|---|---|---|
| Profile | myeloma, kappa secreting, 8-azaguanine resistant, (Lou rat) | Other Name | |
| Animal | rat | Strain | |
| Genus | Rattus | Species | norvegicus |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | hemo-lymphocytic | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | myeloma, kappa secreting | |
| Life Span | Crisis PDL | ||
| Morphology | Character | myeloma, kappa secreting | |
| Classify | Established by | Galfre,G. et al. | |
| Registered by | Regulation for Distribution | ||
| Comment | Prepared from CRL1631. | Year | 1986 |
| Medium | Dulbecco's modified Eagle's medium with 4.5g/L glucose and 5% fetal calf serum. | Methods for Passages | Dilution. A maximum of 1-2 x 10^6/ml is obtainable. |
| Cell Number on Passage | 1-2 x 10^5/ml | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | myeloma |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:310519 | Rat x rat hybrid myelomas and a monoclonal anti-Fd portion of mouse IgG. Galfrè G,Milstein C,Wright B Nature. 1979 Jan 11;277(5692):131-3 |
| Pubmed id:4668496 | Transplantable immunoglobulin-secreting tumours in rats. I. General features of LOU-Ws1 strain rat immunocytomas and their monoclonal proteins. Bazin H,Deckers C,Beckers A,Heremans JF Int J Cancer. 1972 Nov;10(3):568-80 |
LOT Information
| Cell No. | JCRB9091 | Cell Name | Y3-Ag 1.2.3 |
|---|---|---|---|
| LOT No. | 020289 | Lot Specification | distribution |
| Medium | DMEM w/ 4.5g/L glucose + FCS 5% | Temperature | 37 |
| Cell Density at Seeding | 1-2 x 10^5/ml | Methods for Passages | Dilution. A maximum of 1-2 x 10^6/ml is obtainable. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 7.8x10^5 |
| Viability at cell freezing (%) | 98% | Antibiotics Used | free |
| Passage Number | *P4 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | Chromosome Information | NT | |
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB9091 | Cell Name | |
|---|---|---|---|
| LOT No. | 081197 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 4.5 g/L glucose and 5% FCS (lot; BioWhittaker 6M0121) | Temperature | 37 C |
| Cell Density at Seeding | 0.5-1.0 x 10^5 cells/ml | Methods for Passages | Dilution |
| Doubling Time | Cell Number in Vial (cells/1ml) | 6.1 x 10^6 | |
| Viability at cell freezing (%) | 91.0 | Antibiotics Used | free |
| Passage Number | P5* | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | confirmed as rat by NP, G6PD, AST. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | No | Exoteric Gene | |
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |