JCRB9093 WEHI-3
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB9093 | Cell Name | WEHI-3 |
|---|---|---|---|
| Profile | leukemia, myelomonocytic, macrophage-like, (BALB/c mouse) | Other Name | |
| Animal | mouse | Strain | |
| Genus | Mus | Species | musclus |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | hemo-lymphocytic | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | myelomonocytic leukemia, macrophage-like | |
| Life Span | Crisis PDL | ||
| Morphology | Character | myelomonocytic leukemia, macrophage-like | |
| Classify | Established by | ||
| Registered by | Regulation for Distribution | ||
| Comment | Prepared from TIB 68. | Year | 1987 |
| Medium | Dulbecco's modified Eagle's medium with 10% fetal calf serum. | Methods for Passages | Essentially suspension culture. Some adherent cells can be dislodged by scraping. A saturation density of 1-2 x 10^6/ml is obtainable. |
| Cell Number on Passage | 1-2 x 10^5/ml | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | peripheral blood |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:2970637 | Purification and partial characterization of a receptor protein for mouse interferon gamma. Basu M,Pace JL,Pinson DM,Hayes MP,Trotta PP,Russell SW Proc Natl Acad Sci U S A. 1988 Sep;85(17):6282-6 |
| Pubmed id:6170720 | Major histocompatibility complex-restricted antigen presentation to antigen-reactive T cells by B lymphocyte tumor cells. McKean DJ,Infante AJ,Nilson A,Kimoto M,Fathman CG,Walker E,Warner N J Exp Med. 1981 Nov 1;154(5):1419-31 |
| Pubmed id:894031 | Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. Ralph P,Nakoinz I J Immunol. 1977 Sep;119(3):950-54 |
| Pubmed id:318922 | Direct toxic effects of immunopotentiators on monocytic, myelomonocytic, and histiocytic or macrophage tumor cells in culture. Ralph P,Nakoinz I Cancer Res. 1977 Feb;37(2):546-50 |
| Pubmed id:5259326 | Colony formation in vitro by myelomonocytic leukemic cells. Metcalf D,Moore MA,Warner NL J Natl Cancer Inst. 1969 Oct;43(4):983-1001 |
| Images |
|---|
  |
LOT Information
| Cell No. | JCRB9093 | Cell Name | WEHI-3 |
|---|---|---|---|
| LOT No. | 070392 | Lot Specification | distribution |
| Medium | Dulbecco's E.MEM(lot.041592,051592) + 10% FBS (lot.Cell Culture Laboratories,00939-01,JRS.DO10400) | Temperature | 37 C |
| Cell Density at Seeding | 1-3x10^6 | Methods for Passages | Harvest with rubber policeman if adherent cells were appeared. Basically dilution is applied. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 4.7x10^6 |
| Viability at cell freezing (%) | 68 % | Antibiotics Used | free |
| Passage Number | *P5 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB9093 | Cell Name | WEHI-3 |
|---|---|---|---|
| LOT No. | 111888 | Lot Specification | distribution |
| Medium | DMEM + FCS 10% | Temperature | 37 |
| Cell Density at Seeding | 1-2 x 10^5/ml | Methods for Passages | Adherent cells can be dislodged by scraping. A saturation density of 1-2 x 10^6/ml is obtainable. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.1x10^6 |
| Viability at cell freezing (%) | NT | Antibiotics Used | free |
| Passage Number | *P4 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | Chromosome Information | NT | |
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB9093 | Cell Name | |
|---|---|---|---|
| LOT No. | 120597 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121) | Temperature | 37 C |
| Cell Density at Seeding | 3-5 x 10^4 cells/ml | Methods for Passages | dilution |
| Doubling Time | Cell Number in Vial (cells/1ml) | 4.4 x 10^6 | |
| Viability at cell freezing (%) | 90.0 | Antibiotics Used | free |
| Passage Number | P8* | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as mouse by NP, G6PD, AST. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | No | Exoteric Gene | |
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |