JCRB9099 SV-T2
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB9099 | Cell Name | SV-T2 |
|---|---|---|---|
| Profile | SV40 virus-transformed BALB/3T3, (BALB/c mouse) | Other Name | |
| Animal | mouse | Strain | BALB/C |
| Genus | Mus | Species | musclus |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | embryo | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | SV40 virus-transformed BALB/3T3, embryo | |
| Life Span | Crisis PDL | ||
| Morphology | Character | SV40 virus-transformed BALB/3T3, embryo | |
| Classify | Established by | Aaronson & Todaro | |
| Registered by | Regulation for Distribution | ||
| Comment | Prepared from CCL163.1. | Year | 1986 |
| Medium | DMEM + CS 10% | Methods for Passages | 0.02% EDTA and 0.1% trypsin. |
| Cell Number on Passage | 1/4 - 1/10 | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | embryo |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:4301647 | Basis for the acquisition of malignant potential by mouse cells cultivated in vitro. Aaronson SA,Todaro GJ Science. 1968 Nov 29;162(3857):1024-6 |
| Pubmed id:4301006 | Development of 3T3-like lines from Balb-c mouse embryo cultures: transformation susceptibility to SV40. Aaronson SA,Todaro GJ J Cell Physiol. 1968 Oct;72(2):141-8 |
LOT Information
| Cell No. | JCRB9099 | Cell Name | |
|---|---|---|---|
| LOT No. | 071499 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121) | Temperature | 37 C |
| Cell Density at Seeding | 1.5 x 10^5 cells/ml | Methods for Passages | 0.1% trypsin and 0.02% EDTA |
| Doubling Time | Cell Number in Vial (cells/1ml) | 1.2 x 10^6 | |
| Viability at cell freezing (%) | 94 | Antibiotics Used | free |
| Passage Number | P162 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as mouse by NP, G6PD AST |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | DMEM with 10% DMSO and 20% FCS | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB9099 | Cell Name | SV-T2 |
|---|---|---|---|
| LOT No. | 041792 | Lot Specification | distribution |
| Medium | D.MEM (lot.030292) + 10% FBS (lot.J.R.Sci.DO10400) | Temperature | 37 C |
| Cell Density at Seeding | 1/4-1/10 | Methods for Passages | 0.1 % trypsin in 0.02 % EDTA |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 9.96x10^6 |
| Viability at cell freezing (%) | 88.9 | Antibiotics Used | free |
| Passage Number | P162 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Exoteric Gene | NT | |
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |