JCRB9107 P3/NS1/1-Ag4-1(NS-1)
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB9107 | Cell Name | P3/NS1/1-Ag4-1(NS-1) |
|---|---|---|---|
| Profile | myeloma, plasmacytoma, non-secreting, 8-azaguanine resistant | Other Name | |
| Animal | mouse | Strain | |
| Genus | Mus | Species | musclus |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | hemo-lymphocytic | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | non-secreting myeloma | |
| Life Span | Crisis PDL | ||
| Morphology | Character | non-secreting myeloma | |
| Classify | Established by | Kohler,G. et al. | |
| Registered by | Regulation for Distribution | ||
| Comment | Prepared from TIB 18. | Year | 1987 |
| Medium | Dulbecco's modified Eagle's medium with 4.5g/L glucose and 10% fetal calf serum. | Methods for Passages | Simple dilution. |
| Cell Number on Passage | 10^5 cells/ml | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | plasmacytoma |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:825377 | Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion. Köhler G,Milstein C Eur J Immunol. 1976 Jul;6(7):511-9 |
| Pubmed id:61874 | Idiotype suppression. II. Amplification of a suppressor T cell with anti-idiotypic activity. Eichmann K Eur J Immunol. 1975 Aug;5(8):511-7 |
| Pubmed id:4449137 | Intracellular immunoglobulin chain synthesis in non-secreting variants of a mouse myeloma: detection of inactive light-chain messenger RNA. Cowan NJ,Secher DS,Milstein C J Mol Biol. 1974 Dec 25;90(4):691-701 |
LOT Information
| Cell No. | JCRB9107 | Cell Name | P3/NS1/1-Ag4-1(NS-1) |
|---|---|---|---|
| LOT No. | 021492 | Lot Specification | distribution |
| Medium | D.MEM + FBS 10% (J.R.Scientific, lot.CO19407) D.MEM(lot.120191 with glucose 4.5g/l) | Temperature | 37 C |
| Cell Density at Seeding | dil.1/2-3 | Methods for Passages | dilution |
| Doubling Time | 24 hr | Cell Number in Vial (cells/1ml) | 5.4x10^6 |
| Viability at cell freezing (%) | 69.1 | Antibiotics Used | free |
| Passage Number | P5* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Exoteric Gene | NT | |
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB9107 | Cell Name | P3/NS1/1-Ag4-1(NS-1) |
|---|---|---|---|
| LOT No. | 03212013 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium (high glucose, 4.5 g/L glucose) with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099) | Temperature | 37 C |
| Cell Density at Seeding | 1 - 4 x 10^5 cells/mL | Methods for Passages | Simple dilution. |
| Doubling Time | Cell Number in Vial (cells/1ml) | 7.9 x 10^5 | |
| Viability at cell freezing (%) | 71.8 | Antibiotics Used | free |
| Passage Number | Unknown (5 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as mouse by NP, G6PD, MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | No | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - DMEM (high glucose) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 71.8 | Additional information |