JCRB9114 BRL-3A

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9114 Cell Name   BRL-3A
Profile   MSA (somatomedin-like) protein producing, (Buffalo rat) Other Name   
Animal   rat Strain   Buffalo
Genus   Rattus Species   norvegicus
Sex    Age   
Identity    Tissue for Primary Cancer   liver
Case history    Metastasis   
Tissue Metastasized    Genetics   liver
Life Span    Crisis PDL   
Morphology    Character   liver producing MSA(somatomedin-like) protein
Classify    Established by   Coon,H.G.
Registered by    Regulation for Distribution   
Comment   Additional Information - modified Eagle's medium with 10 or 20% tryptose phosphate broth (2). Dulbecco-Vogt (3). Year   1986
Medium   Coon's modified Ham's F12 medium(Sigma Co.) with 5% fetal bovine serum Methods for Passages   Cells are treated with 0.25% trypsin and 0.02% EDTA.
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   liver
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0606
Reference
Pubmed id:4308097A quantitative comparison of formation of spontaneous and virus-produced viable hybrids.
Coon HG,Weiss MC
Proc Natl Acad Sci U S A. 1969 Mar;62(3):852-9

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LOT Information

Cell No.   JCRB9114 Cell Name   BRL-3A
LOT No.   102495 Lot Specification   distribution
Medium   Coon's modified Ham's F-12 medium (Sigma Co.) with 5% heat inactivated fetal bovine serum (ICN Flow) Temperature   37 C
Cell Density at Seeding   1.6x10^4 cells/sq.cm. Methods for Passages   Cells are rinced with PBS(-) and treated with 0.25% trypsin-0.02% EDTA solution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   6.1x10^6
Viability at cell freezing (%)   98.4 Antibiotics Used   free
Passage Number   P5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture meditum with 5% DMSO. CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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