JCRB9115 SW837

Cell information

Important Notice(s)

Leibovitz's L-15 medium

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9115 Cell Name   SW837
Profile   adenocarcinoma Other Name   SW837
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   rectum
Case history    Metastasis   
Tissue Metastasized    Genetics   Rectum, adenocarcinoma
Life Span    Crisis PDL   
Morphology    Character   Rectum, adenocarcinoma
Classify    Established by   Leibovitz,A.
Registered by    Regulation for Distribution   ATCC
Comment    Year   1988
Medium   Leibovitz's L-15 medium with 10% fetal calf serum. No CO2 applied. Methods for Passages   0.02% EDTA and 0.25% trypsin. Cells are slow in attaching to substrate.
Cell Number on Passage   1/2 - 1/5 Race   
CO2 Conc.   - Tissue Sampling   rectum
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1729
Reference
Pubmed id:1980764Expression of several growth factors and their receptor genes in human colon carcinomas.
Ito M,Yoshida K,Kyo E,Ayhan A,Nakayama H,Yasui W,Ito H,Tahara E
Virchows Arch B Cell Pathol Incl Mol Pathol. 1990;59(3):173-8
Pubmed id:1000501Classification of human colorectal adenocarcinoma cell lines.
Leibovitz A,Stinson JC,McCombs WB 3rd,McCoy CE,Mazur KC,Mabry ND
Cancer Res. 1976 Dec;36(12):4562-9

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LOT Information

Cell No.   JCRB9115 Cell Name   
LOT No.   11042004 Lot Specification   distribution
Medium   Leibovitz's L-15 medium with 10% fetal bovine serum (FBS lot; GIBCO 3762782S). Cultivate under normal atmosphere (not under 5% CO2). Temperature   37 C
Cell Density at Seeding   3 - 4 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Subcultivate about once a week. Growth of this cell line is slow. The attachment after subcultivation is poor and it takes a few days to form a monolayer.
Doubling Time   ca. 3 days Cell Number in Vial (cells/1ml)   6.2 x 10^6
Viability at cell freezing (%)   81.9 Antibiotics Used   free
Passage Number   P45 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - L15 medium CO2 Conc.   No
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9115 Cell Name   SW837
LOT No.   092194 Lot Specification   FDSC/distribution
Medium   Leibovitz's L15 medium with 10% fetal bovine serum(Bio Cell, 4001776) Temperature   37 C
Cell Density at Seeding   4-9x10^4 cells/ml Methods for Passages   Cells are treated with 0.25% trypsin and harvested.
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.20x10^6
Viability at cell freezing (%)   98.1 Antibiotics Used   free
Passage Number   P44 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Human(NP,G6PD,PepB,MPI,AST,LDH)
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   0 %
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9115 Cell Name   SW837
LOT No.   032089 Lot Specification   distribution
Medium   Leibovitz's L-15 medium + FCS 10% Temperature   37 C
Cell Density at Seeding   1/2 - 1/5 Methods for Passages   0.02% EDTA and 0.25% trypsin. Cells are slow in attaching to substrate.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.9x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P44 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   0 %
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9115 Cell Name   SW837
LOT No.   04122024 Lot Specification   distribution
Medium   Leibovitz's L-15 medium with 10% fetal bovine serum (FBS lot; Nichirei Cat. # 174112, Lot 20M00K). Cultivate under normal atmosphere (not 5% CO2). Temperature   37 C
Cell Density at Seeding   1.3 - 2.4 x 10^5 cells/mL Methods for Passages   0.25% trypsin and 0.02% EDTA. The attachment after subcultivation is poor and it takes a few days to form a monolayer.
Doubling Time   approx. 49 hrs. Cell Number in Vial (cells/1ml)   2.9 x 10^6
Viability at cell freezing (%)   97 Antibiotics Used   free
Passage Number   P45 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - L-15 CO2 Conc.   No
Viability immediately after thawing (%)   97 Additional information   
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