JCRB0211 A9

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0211 Cell Name   A9
Profile   skin fibroblast, 8-azaguanine-resistant, (C3H mouse) Other Name   A9
Animal   mouse Strain   C3H
Genus   Mus Species   musculus
Sex    Age   
Identity   not done Tissue for Primary Cancer   skin
Case history    Metastasis   
Tissue Metastasized    Genetics   HPRT and APRT deficient.
Life Span   infinite Crisis PDL   
Morphology    Character   skin fibroblast, AG-r
Classify    Established by   Littlefield,J.W.
Registered by   Isaka,H. Regulation for Distribution   
Comment   Resistant to 8-azaguanine. Year   1985
Medium   Eagle's minimal essential medium with 10% fetal calf serum Methods for Passages   Cells are treated with 0.25% trypsin.
Cell Number on Passage   2 x 10^4 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   skin
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3984
Reference
Pubmed id:14213660THREE DEGREES OF GUANYLIC ACID--INOSINIC ACID PYROPHOSPHORYLASE DEFICIENCY IN MOUSE FIBROBLASTS.
LITTLEFIELD JW
Nature. 1964 Sep 12;203():1142-4

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LOT Information

Cell No.   JCRB0211 Cell Name   A9
LOT No.   07122006 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum (FBS GIBCO-catalog#10099, lot4218333S) Temperature   37 C
Cell Density at Seeding   2 - 10 x 10^4 cells/ml Methods for Passages   0.25% trypsin
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   4.1 x 10^6
Viability at cell freezing (%)   98.2 Antibiotics Used   free
Passage Number   P7 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0211 Cell Name   
LOT No.   031792 Lot Specification   distribution
Medium   MEM : 10%FCS Temperature   37
Cell Density at Seeding    Methods for Passages   
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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