JCRB0308 AT1OS
Cell information
Cell type：human mutant cell repository from Kyoto University (View Pricing Information)
| JCRB No. | JCRB0308 | Cell Name | AT1OS |
|---|---|---|---|
| Profile | Hereditary disease with high cancer risk, ataxia telangiectasia (autosomal recessive), chromosome breakage syndrome, skin fibroblast, high sensitivity to X-ray. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | ||
| Identity | available | Tissue for Primary Cancer | skin |
| Case history | ataxia telangiectasia | Metastasis | |
| Tissue Metastasized | Genetics | Ataxia telangiectasia, fibroblast | |
| Life Span | Crisis PDL | ||
| Morphology | fibroblast-like | Character | Ataxia telangiectasia, fibroblast |
| Classify | Established by | Ikenaga,M. | |
| Registered by | Ikenaga,M. | Regulation for Distribution | |
| Comment | Now new stock of the AT1OS available (03/12/2004). | Year | 1985 |
| Medium | RPMI1640 medium, 20% non-heat-inactivated fetal bovine serum. (establish: Dulbecco's modified Eagle's medium with 10% fetal calf serum) | Methods for Passages | trypsin |
| Cell Number on Passage | split ratio=1/3 | Race | Japanese |
| CO2 Conc. | 5 % | Tissue Sampling | skin |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | not tested | ||||||
| CMV | - | parvoB19 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| EBV | - | HBV | - | ||||||
| HHV6 | - | HTLV-1 | - | ||||||
| HHV7 | - | HTLV-2 | - | ||||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:2465834 | Replicon initiation in normal human cells and in ataxia telangiectasia cells: its differential inhibition by cycloheximide and bleomycin. Noda A Cell Biol Int Rep. 1988 Nov;12(11):943-50 |
| Pubmed id:6876478 | The sensitivities to radiations and radiomimetic chemicals of cells from patients with ataxia telangiectasia. Ikenaga M,Midorikawa M,Abe J,Mimaki T Jinrui Idengaku Zasshi. 1983 Mar;28(1):1-10 |
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LOT Information
| Cell No. | JCRB0308 | Cell Name | AT1OS |
|---|---|---|---|
| LOT No. | 10082008 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO) with 20% bovine fetal serum(Hyclone GRD0051,non HI) | Temperature | 37 C |
| Cell Density at Seeding | 1.0x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and PBS. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 8.0x10^4 |
| Viability at cell freezing (%) | 99.5 | Antibiotics Used | free |
| Passage Number | p14 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:10 D13S317:9 D7S820:11,12 D16S539:9,12 VWA:17,18 TH01:9 AM:X,Y TPOX:10,11 CSF1PO:10,12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB0308 | Cell Name | |
|---|---|---|---|
| LOT No. | 02-01-28 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 10% fetal calf serum | Temperature | 37 C |
| Cell Density at Seeding | split ratio = 1/3 | Methods for Passages | treatment with 0.25% trypsin and 0.02% EDTA |
| Doubling Time | Cell Number in Vial (cells/1ml) | ||
| Viability at cell freezing (%) | Antibiotics Used | free | |
| Passage Number | P9 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | Culture medium with 5% DMSO | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |