JCRB0310 CS2AW

Cell information

Cell type:human mutant cell repository from Kyoto University (View Pricing Information)

JCRB No.   JCRB0310 Cell Name   CS2AW
Profile   Hereditary disease showing accelerated aging Cockayne syndrome, group(A), (autosomal recessive) skin fibroblast, high sensitivity to UV. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   5Y
Identity   available Tissue for Primary Cancer   skin
Case history   Cockayne syndrome, group(A) Metastasis   
Tissue Metastasized    Genetics   Group A Cockayne syndrome.
Life Span   finite Crisis PDL   
Morphology   fibroblast-like Character   Cockayne syndrome cells without XP phenotype. group(A)
Classify   primary Established by   Ikenaga,M.
Registered by   Ikenaga,M. Regulation for Distribution   
Comment   Hereditary disease showing accelerated aging,Cockayne syndrome (autosomal recessive),skin fibroblast,high sensitivity to UV. Year   1985
Medium   RPMI1640 medium, 20% non-heat-inactivated fetal bovine serum. (establish: Dulbecco's modified Eagle's medium with 10% fetal calf serum.) Methods for Passages   trypsin
Cell Number on Passage   split ratio=1/3 Race   
CO2 Conc.   5 % Tissue Sampling   skin
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2879
Reference
Pubmed id:noneCockayne症候群:姉妹例の報告と本邦における文献的展望
徳島大学医学部小児科:橋本俊顕ら
脳と発達 10(6):465-472,1978

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LOT Information

Cell No.   JCRB0310 Cell Name   CS2AW
LOT No.   09112008 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 20% fetal bovine serum(Hyclone GRD0051,non HI) Temperature   37 C
Cell Density at Seeding   1.4x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.1x10^6
Viability at cell freezing (%)   98.4 Antibiotics Used   free
Passage Number   p7 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,13
D13S317:10,12
D7S820:11
D16S539:9,10
VWA:14,17
TH01:7,10
AM:X
TPOX:11
CSF1PO:10,13
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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