JCRB0314 FA9JTO

Cell information

Cell type:human mutant cell repository from Kyoto University (View Pricing Information)

JCRB No.   JCRB0314 Cell Name   FA9JTO
Profile    Hereditary disease with high cancer risk, Fanconi's anemia (autosomal recessive), chromosomal breakage syndrome, high sensitivity to mitomycin C (lymphocyte), skin fibroblast. Other Name   FA9P
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   7
Identity   available Tissue for Primary Cancer   skin
Case history    Metastasis   
Tissue Metastasized    Genetics   MMC sensitive(lymphocyte), FANCG mutation
Life Span    Crisis PDL   
Morphology    Character   Fanconi anemia(G), fibroblast
Classify    Established by   Sasaki,M.
Registered by   Sasaki,M. Regulation for Distribution   
Comment   Hereditary disease with high cancer risk, Fanconi's anemia (autosomal recessive), chromosomal breakage syndrome, high sensitivity to mitomycin C, skin fibroblast. Year   1960
Medium   RPMI1640 medium, 20% non-heat-inactivated fetal bovine serum. (establish: alpha-MEM medium with 20% fetal calf serum) Methods for Passages   0.25% trypsin, sprit ratio = 1/4
Cell Number on Passage   1x10^5 cells/100mm dish Race   
CO2 Conc.   5 % Tissue Sampling   skin
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2896
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LOT Information

Cell No.   JCRB0314 Cell Name   FA9JTO
LOT No.   06062008 Lot Specification   distribution
Medium   RPMI1640(GIBCO) with 20% heat inactivated fetal bovine serum(Hyclone GRD0051) Temperature   37 C
Cell Density at Seeding   4.4x10^3 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.2x10^6
Viability at cell freezing (%)   99.0 Antibiotics Used   free
Passage Number   p8 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12
D13S317:9,12
D7S820:11
D16S539:9,11
VWA:14
TH01:9
AM:X,Y
TPOX:8,11
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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