JCRB0331 AT(L)5KY
Cell information
Cell type：human mutant cell repository from Kyoto University (View Pricing Information)
| JCRB No. | JCRB0331 | Cell Name | AT(L)5KY |
|---|---|---|---|
| Profile | EBV transformed B lymphoblastoid cell line, ataxia telangiectasia | Other Name | AT(L)5KY |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 10 |
| Identity | available | Tissue for Primary Cancer | hemo-lymphocytic |
| Case history | ataxia telangiectasia | Metastasis | No |
| Tissue Metastasized | Genetics | 2n=46,46,XX, inv(9)(pter->p13::q13->p13::q13-qter) | |
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | EBV transformed B cell lymphoblastoid cell line |
| Classify | transformed | Established by | Ejima,Y. |
| Registered by | Ejima,Y. | Regulation for Distribution | JCRB |
| Comment | Year | 1991 | |
| Medium | RPMI1640 medium with 10% fetal calf serum | Methods for Passages | dilution. Split ratio=1/2.5 |
| Cell Number on Passage | 4x10^5 cells/ml | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | peripheral blood |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | + | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
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LOT Information
| Cell No. | JCRB0331 | Cell Name | AT(L)5KY |
|---|---|---|---|
| LOT No. | TEIKEI | Lot Specification | distribution |
| Medium | RPMI 1640 | Temperature | 37 |
| Cell Density at Seeding | 4x10^5/ml | Methods for Passages | dilution. Split ratio=1:2.5 |
| Doubling Time | Cell Number in Vial (cells/1ml) | ||
| Viability at cell freezing (%) | Antibiotics Used | ||
| Passage Number | PDL | ||
| Sterility: MYCOPLASMA | Sterility: BACTERIA | ||
| Sterility: FUNGI | Isozyme Analysis | ||
| Chromosome Mode | 46 | Chromosome Information | 46,XY 2N=46 |
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0331 | Cell Name | AT(L)5KY |
|---|---|---|---|
| LOT No. | 04282009 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum | Temperature | 37 C |
| Cell Density at Seeding | Methods for Passages | Dilution | |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | |
| Viability at cell freezing (%) | 98.4 | Antibiotics Used | free |
| Passage Number | p4* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:10,14 D13S317:9,11 D7S820:11 D16S539:9,10 VWA:14,17 TH01:6,7 AM:X TPOX:8 CSF1PO:11,12 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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