JCRB0333 AT(L)7KY
Cell information
Cell type：human mutant cell repository from Kyoto University (View Pricing Information)
| JCRB No. | JCRB0333 | Cell Name | AT(L)7KY |
|---|---|---|---|
| Profile | EBV transformed B lymphoblastoid cell line, ataxia telangiectasia | Other Name | AT(L)7KY |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 3 |
| Identity | available | Tissue for Primary Cancer | hemo-lymphocytic |
| Case history | ataxia telangiectasia | Metastasis | No |
| Tissue Metastasized | Genetics | 2n=46, 46,XY | |
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | EBV transformed B lymphoblastoid cell line |
| Classify | transformed | Established by | Ejima,Y. |
| Registered by | Ejima,Y. | Regulation for Distribution | |
| Comment | Year | 1991 | |
| Medium | RPMI1640 medium with 10% fetal calf serum | Methods for Passages | suspension culture, split 1/2.5 when subculture |
| Cell Number on Passage | 4x10^5 cells/ml | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | peripheral blood |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | + | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | + | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Images |
|---|
           |
LOT Information
| Cell No. | JCRB0333 | Cell Name | AT(L)7KY |
|---|---|---|---|
| LOT No. | 09172008 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(Hyclone GRD0051) | Temperature | 37 C |
| Cell Density at Seeding | 5.0x10^5 cells/ml | Methods for Passages | dilution (split ratio=1/2.5) |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 3.6x10^6 |
| Viability at cell freezing (%) | 94.0 | Antibiotics Used | free |
| Passage Number | p5* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:9,12 D13S317:8,12 D7S820:11,13 D16S539:10,11 VWA:17,18 TH01:6,9 AM:X,Y TPOX:8 CSF1PO:9,13 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
| Images |
|---|
|
|