JCRB1056 CS2OS(SVT)
Cell information
Cell type：human mutant cell repository from Kyoto University (View Pricing Information)
| JCRB No. | JCRB1056 | Cell Name | CS2OS(SVT) |
|---|---|---|---|
| Profile | SV40 transformed human fibroblast from fetus, Cockayne syndrome, group(A) | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | fetus |
| Identity | available | Tissue for Primary Cancer | skin |
| Case history | Cockayne syndrome, group(A) | Metastasis | |
| Tissue Metastasized | Genetics | Group A Cocayne syndrome. Large chromosome aberrations have not been found. Ori-deficient SV40 transformed. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | fibroblast-like | Character | Fibroblast cells transformed with ori-deficient SV40. |
| Classify | transformed | Established by | Tachibana,A. |
| Registered by | Tachibana,A. | Regulation for Distribution | |
| Comment | Hereditary disease showing accelerated aging, Cockayne syndrome(autosomal recessive), fetal skin fibroblast, high sensitivity to UV determined by prenatal diagnosis. SV40 transformed. | Year | 2002 |
| Medium | Alpha-MEM medium with 10% fetal calf serum. | Methods for Passages | Cells are harvested after treatment with trypsin and EDTA. |
| Cell Number on Passage | split ratio=1/4 | Race | Japanese |
| CO2 Conc. | 5 % | Tissue Sampling | skin |
| Tissue Type | SV40 transformed cells. |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:7151298 | Prenatal diagnosis of Cockayne syndrome using assay of colony-forming ability in ultraviolet light irradiated cells. Sugita T,Ikenaga M,Suehara N,Kozuka T,Furuyama J,Yabuuchi H Clin Genet. 1982 Sep;22(3):137-42 |
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LOT Information
| Cell No. | JCRB1056 | Cell Name | CS2OS(SVT) |
|---|---|---|---|
| LOT No. | 07042003 | Lot Specification | distribution |
| Medium | Alpha-MEM with 10% fetal calf serum(Intergen RB52305). | Temperature | 37 C |
| Cell Density at Seeding | 1.0 x 10^4 cells/sq.cm. | Methods for Passages | Cells harvested after treatment with 0.25% trypsin. Subculture every 4-5 days and medium change every 3-4 days. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.3 x 10^6 |
| Viability at cell freezing (%) | 97.1 | Antibiotics Used | |
| Passage Number | PDL | ||
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | G6PD(typeB), NP, LDH examined. Human not HeLa. |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:13 D13S317:8,9 D7S820:11 D16S539:9 VWA:16 TH01:7 AM:X,Y TPOX:11,13 CSF1PO:11,12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Culture medium with 5% DMSO. | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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