JCRB1075.1 IM95m

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1075.1 Cell Name   IM95m
Profile   Human gastric cancer cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   63
Identity   available Tissue for Primary Cancer   stomach
Case history   moderately differentiated adenocarcinoma of stomach Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Clone from the IM95. A clone from the IM95, produces higher level of HGF & VEGF.
Classify   tumor Established by   Iwai,M. & Matsuda,M.
Registered by   Iwai,M. Regulation for Distribution   
Comment    Year   2003
Medium   Dulbecco's modified Eagle's medium(4.5g/L glucose) with 10% fetal bovine serum and 10mg/L insulin. Methods for Passages   Cells were harvested after the treatment with trypsin-EDTA.
Cell Number on Passage    Race   Japanese
CO2 Conc.   5 % Tissue Sampling   stomach
Tissue Type   stomach cancer

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2962
Reference
Pubmed id:14609362Cloning of a cancer cell-producing hepatocyte growth factor, vascular endothelial growth factor, and interleukin-8 from gastric cancer cells.
Iwai M,Matsuda M,Iwai Y
In Vitro Cell Dev Biol Anim. 2003 Jul-Aug;39(7):288-90

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LOT Information

Cell No.   JCRB1075.1 Cell Name   IM95m
LOT No.   08092011 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (high glucose, 4.5 g/L) with 10 ug/mL insulin and 10% fetal bovine serum (FBS: GIBCO Cat.# 10099) Temperature   37 C
Cell Density at Seeding   5.2 -5.7 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Passaged twice a week.
Doubling Time   approx. 39 hrs. Cell Number in Vial (cells/1ml)   6.6 x 10^6
Viability at cell freezing (%)   97.0 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:9,11
D13S317:10,12
D7S820:9.3
D16S539:9,10
VWA:17,18
TH01:6,10
AM:X
TPOX:8,10
CSF1PO:11
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM (high glucose) CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1075.1 Cell Name   
LOT No.   02062006 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (4.5 g/L glucose) with 10 ug/ml insulin and 10% fetal bovine serum (FBS Lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   2-3 x 10^5 cells/ml Methods for Passages   Harvested with 0.25% trypsin and 0.02% EDTA (could not completely dissociate into single cell suspension). Medium renewal every 2-3 days.
Doubling Time   approx. 39 hrs Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   98.6 Antibiotics Used   free
Passage Number   Unknown (P6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
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