JCRB1363 iPS-TIG120-4f1
Cell information
Cell type：human iPS cells (View Pricing Information)
| JCRB No. | JCRB1363 | Cell Name | iPS-TIG120-4f1 |
|---|---|---|---|
| Profile | Human iPS cell line which was generated from adult human skin fibroblast cell line (JCRB0542: TIG-120). | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 6 |
| Identity | available | Tissue for Primary Cancer | |
| Case history | Metastasis | No | |
| Tissue Metastasized | Genetics | Transgene: Human OCT3/4, SOX2, KLF4, and c-MYC (in pMXs retroviral vectors). | |
| Life Span | infinite | Crisis PDL | |
| Morphology | human ES-like | Character | |
| Classify | transformed | Established by | Yamanaka, S. |
| Registered by | Yamanaka, S. | Regulation for Distribution | 1) Academic research purpose only. 2) Conclude a MTA with Kyoto Univ.. |
| Comment | Year | 2009 | |
| Medium | Primate ES cells medium (ReproCELL) supplemented 4 ng/mL bFGF. | Methods for Passages | Cells are treated with the solution consiseted by 0.25% Trypsin, 0.1 mg/mL collagenase IV, 1mM CaCl2, and 20% KSR. |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | skin |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:22119741 | Screening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage. International Stem Cell Initiative.,Amps K,Andrews PW,Anyfantis G,Armstrong L,Avery S,Baharvand H,Baker J,Baker D,Munoz MB,Beil S,Benvenisty N,Ben-Yosef D,Biancotti JC,Bosman A,Brena RM,Brison D,Caisander G,Camarasa MV,Chen J,Chiao E,Choi YM,Choo AB,Collins D,Colman A,Crook JM,Daley GQ,Dalton A,De Sousa PA,Denning C,Downie J,Dvorak P,Montgomery KD,Feki A,Ford A,Fox V,Fraga AM,Frumkin T,Ge L,Gokhale PJ,Golan-Lev T,Gourabi H,Gropp M,Lu G,Hampl A,Harron K,Healy L,Herath W,Holm F,Hovatta O,Hyllner J,Inamdar MS,Irwanto AK,Ishii T,Jaconi M,Jin Y,Kimber S,Kiselev S,Knowles BB,Kopper O,Kukharenko V,Kuliev A,Lagarkova MA,Laird PW,Lako M,Laslett AL,Lavon N,Lee DR,Lee JE,Li C,Lim LS,Ludwig TE,Ma Y,Maltby E,Mateizel I,Mayshar Y,Mileikovsky M,Minger SL,Miyazaki T,Moon SY,Moore H,Mummery C,Nagy A,Nakatsuji N,Narwani K,Oh SK,Oh SK,Olson C,Otonkoski T,Pan F,Park IH,Pells S,Pera MF,Pereira LV,Qi O,Raj GS,Reubinoff B,Robins A,Robson P,Rossant J,Salekdeh GH,Schulz TC,Sermon K,Sheik Mohamed J,Shen H,Sherrer E,Sidhu K,Sivarajah S,Skottman H,Spits C,Stacey GN,Strehl R,Strelchenko N,Suemori H,Sun B,Suuronen R,Takahashi K,Tuuri T,Venu P,Verlinsky Y,Ward-van Oostwaard D,Weisenberger DJ,Wu Y,Yamanaka S,Young L,Zhou Q Nat Biotechnol. 2011 Nov 27;29(12):1132-44 |
| Pubmed id:18035408 | Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Takahashi K,Tanabe K,Ohnuki M,Narita M,Ichisaka T,Tomoda K,Yamanaka S Cell. 2007 Nov 30;131(5):861-72 |
| Images |
|---|
         |
| PDF File1 PDF File2 |
LOT Information
| Cell No. | JCRB1363 | Cell Name | iPS-TIG120-4f1 |
|---|---|---|---|
| LOT No. | 06302010 | Lot Specification | distribution |
| Medium | culture medium for human ES cells (attachmentA-1)+FGF-2 4ng/ml | Temperature | 37 C |
| Cell Density at Seeding | 1/2-1/6 split | Methods for Passages | 0.1% Dispase(Dispase II neutral protease,grade II,Roche) |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | |
| Viability at cell freezing (%) | Antibiotics Used | free | |
| Passage Number | p7+26 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:9,11 D13S317:10 D7S820:10,12 D16S539:12 VWA:14,20 TH01:9,9.3 AM:X TPOX:8,11 CSF1PO:12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | culture medium containing 10% DMSO | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
| Images |
|---|
|
|
| Cell No. | JCRB1363 | Cell Name | iPS-TIG120-4f1 |
|---|---|---|---|
| LOT No. | 06252010 | Lot Specification | distribution |
| Medium | culture medium for human ES cells (attachmentA-1)+FGF-2 4ng/ml | Temperature | 37 C |
| Cell Density at Seeding | 1/2-1/6 split | Methods for Passages | 0.1% Dispase(Dispase II neutral protease,grade II,Roche) |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | |
| Viability at cell freezing (%) | Antibiotics Used | free | |
| Passage Number | p7+25 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | 46 | Chromosome Information | 46,XX |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:9,11 D13S317:10 D7S820:10,12 D16S539:12 VWA:14,20 TH01:9,9.3 AM:X TPOX:8,11 CSF1PO:12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | culture medium containing 10% DMSO | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
| Images |
|---|
 |
| PDF File1 |