JCRB1465 B16F10-Luc 1-#3
Cell information
Important Notice(s)B16F10-Luc 1-#3: Low Luc expression level.
発光細胞 (ルシフェラーゼ発現細胞)の分譲について / Ordering Luc cell lines
Information for introduced genes, etc in luciferase expressing cell lines
Cell type：luciferase-expressing cancer cells (View Pricing Information)
| JCRB No. | JCRB1465 | Cell Name | B16F10-Luc 1-#3 |
|---|---|---|---|
| Profile | Luciferase stably expressing cell line (B16-F10; Mouse melanoma cell line). The problem in luminescence was found and the distribution is suspended. Please consider to use JCRB1675 B16-F10/CMV-Luc#2. | Other Name | |
| Animal | mouse | Strain | C57BL/6J |
| Genus | Mus | Species | musclus |
| Sex | Age | ||
| Identity | not done | Tissue for Primary Cancer | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | pMSCV-luc transfected. Luciferase stably expressing cell line. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | spindle-shape | Character | puromycin-resistant |
| Classify | transformed | Established by | Murakami, T. |
| Registered by | Murakami, T. | Regulation for Distribution | To exchange and follow MTA between oversea end-user and cell-developer. |
| Comment | Year | 2011 | |
| Medium | DMEM medium with 10% fetal bovine serum. | Methods for Passages | Cells are treated with 0.02 % EDTA and 0.25% trypsin. |
| Cell Number on Passage | split ratio = 1/4 - 1/10 | Race | |
| CO2 Conc. | 5% | Tissue Sampling | skin |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
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LOT Information
| Cell No. | JCRB1465 | Cell Name | B16F10-Luc 1-#3 |
|---|---|---|---|
| LOT No. | 12042012 | Lot Specification | distribution |
| Medium | DMEM with 10% heat inactivated fetal bovine serum(SIGMA027K03911) | Temperature | 37 C |
| Cell Density at Seeding | 0.5-5.8x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.(Split ratio=1/4-1/10) |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.63x10^6 |
| Viability at cell freezing (%) | 96.4 | Antibiotics Used | free |
| Passage Number | p10* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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