JCRB1618 AgMES

Cell information

Cell type：primate ES/iPS cells (View Pricing Information)

JCRB No.   JCRB1618 Cell Name   AgMES
Profile   ES cell line established from African Green Monkey. Other Name   
Animal   monkey, African green Strain   
Genus   Cercopithecus Species   aethiops
Sex   F Age   
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   No
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   ES-like Character   
Classify   transformed Established by   Shimozawa N.
Registered by   Shimozawa N. Regulation for Distribution   Restricted by Washington Treaty
Comment    Year   2010
Medium   DMEM/F12 (1:1) supplemented with 20% KSR, 1% GlutaMax, 0.1 mM 2-ME, 1% NEAA, and 10 ng/mL hLIF. Methods for Passages   0.1% collagenase in DMEM and divided mechanically into small clusters with pipetting.
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_RY74



LOT Information

Cell No.   JCRB1618 Cell Name   AgMES
LOT No.   03012015 Lot Specification   distribution
Medium   DMEM and Ham's F12 medium(1/1) with 20% SSR(Wako),1% GlutaMax,1% NEAA,0.1mM 2-mercaptoethanol,10ng/ml hu r LIF on mitomycin C-treated MEF(ICR) feeder cells Temperature   37 C
Cell Density at Seeding   NT Methods for Passages   Cells were treated with 0.1% collagenase in DMEM and divided mechanically into small clusters with pipetting.(1/6-1/10 Split)
Doubling Time   NT Cell Number in Vial (cells/1ml)   uncountable
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   p18 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   STEM-CELLBANKER(ZENOAQ) CO2 Conc.   5%
Viability immediately after thawing (%)   NT Additional information   Mitomycin C -treated MEFs are used as feeders. 1vial is 400ul.Type of ampoules is plastic.
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