JCRB0832 NH-6
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0832 | Cell Name | NH-6 |
|---|---|---|---|
| Profile | neuroblastoma, adrenal gland, opsomyoclonus, truncal ataxia | Other Name | neuroblastoma-6 |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 9-month-old |
| Identity | available | Tissue for Primary Cancer | neural |
| Case history | opsomyoclonus,truncal ataxia | Metastasis | Yes |
| Tissue Metastasized | lymph node | Genetics | |
| Life Span | infinite | Crisis PDL | |
| Morphology | fibroblast-like | Character | neuroblastoma cell line from primary tumor |
| Classify | tumor | Established by | Hiyama,E. & Nakamura,N. |
| Registered by | Akiyama,M. | Regulation for Distribution | |
| Comment | Year | 1992 | |
| Medium | MEM-alpha with 10% fetal calf serum. | Methods for Passages | Cells are treated with 0.25% trypsin and 0.02% EDTA and dilute in fresh medium. |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | adrenal gland |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | not tested | ||||||
| CMV | - | parvoB19 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| EBV | - | HBV | - | ||||||
| HHV6 | - | HTLV-1 | - | ||||||
| HHV7 | - | HTLV-2 | - | ||||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | NT | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:2004381 | Neuroblastoma with DNA amplification and rearrangement in the N-myc gene region. Hiyama E,Hiyama K,Yokoyama T Cancer Res. 1991 Apr 1;51(7):1946-51 |
| Images |
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LOT Information
| Cell No. | JCRB0832 | Cell Name | NH-6 |
|---|---|---|---|
| LOT No. | 06262000 | Lot Specification | distribution |
| Medium | alpha-MEM with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099) | Temperature | 37 C |
| Cell Density at Seeding | 6-8 x 10^4 (do not exceed 10^5 cells/ml) | Methods for Passages | 0.25% trypsin and 0.02% EDTA. The growth of NH-6 cells tends to stop before confluency, and should be passaged when the culture reachs to 50% confluency. |
| Doubling Time | > 3days | Cell Number in Vial (cells/1ml) | 1.7 x 10^6 |
| Viability at cell freezing (%) | 92 | Antibiotics Used | free |
| Passage Number | P30 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), AST. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | Culture medium containing 10% DMSO, 20% FCS | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information | Reg.No. is mislabeled as JCRC0832. Correct No. is JCRB0832. |
| Cell No. | JCRB0832 | Cell Name | |
|---|---|---|---|
| LOT No. | 091395 | Lot Specification | distribution |
| Medium | alpha MEM + 10% FCS | Temperature | 37 C |
| Cell Density at Seeding | Methods for Passages | ||
| Doubling Time | Cell Number in Vial (cells/1ml) | ||
| Viability at cell freezing (%) | Antibiotics Used | ||
| Passage Number | PDL | ||
| Sterility: MYCOPLASMA | Sterility: BACTERIA | ||
| Sterility: FUNGI | Isozyme Analysis | ||
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |