JCRB9122 SIRC

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9122 Cell Name   SIRC
Profile   susceptible to rubella virus Other Name   
Animal   rabbit Strain   
Genus   Oryctolagus Species   cuniculus
Sex    Age   
Identity    Tissue for Primary Cancer   cornea
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   susceptible to rubella virus, toxicologcal usage
Classify   normal Established by   Not known.
Registered by   NIHS Regulation for Distribution   
Comment    Year   1992
Medium    Methods for Passages   
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   cornea
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2724
Reference
Pubmed id:6042441Plaque assays of rubella virus in cultures of rabbit cornea (SIRC) cells.
Rhim JS,Schell K,Huebner RJ
Proc Soc Exp Biol Med. 1967 Aug-Sep;125(4):1271-4
Pubmed id:5918951Isolation, propagation and neutralization of rubella virus in cultures of rabbit cornea (SIRC) cells.
Phillips CA,Melnick JL,Burkhardt M
Proc Soc Exp Biol Med. 1966 Jul;122(3):783-6
Pubmed id:14331182CYTOPATHIC EFFECT OF RUBELLA VIRUS IN A RABBIT-CORNEA CELL LINE.
LEERHOY J
Science. 1965 Aug 6;149(3684):633-4

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LOT Information

Cell No.   JCRB9122 Cell Name   
LOT No.   11132012 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Passage twice a week.
Doubling Time   approx. 31 hrs Cell Number in Vial (cells/1ml)   2.9 x 10^6
Viability at cell freezing (%)   97.4 Antibiotics Used   free
Passage Number   P417 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as rabbit by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9122 Cell Name   SIRC
LOT No.   091492 Lot Specification   distribution
Medium   Eagle's minimal essential medium (070992) with 10%fetal bovine serum heat inactivated (Flow0070893) Temperature   37 C
Cell Density at Seeding   1.5x10^4 cells/ml Methods for Passages   Treat cells with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.1x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P417 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9122 Cell Name   SIRC
LOT No.   10272017 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.5 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 1.5 days Cell Number in Vial (cells/1ml)   2.4 x 10^6
Viability at cell freezing (%)   95.2 Antibiotics Used   free
Passage Number   P417 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   95.2 Additional information   
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