JCRB9123 B95-8
Cell information
Important Notice(s)Cell type：virus production cells (View Pricing Information)
| JCRB No. | JCRB9123 | Cell Name | B95-8 |
|---|---|---|---|
| Profile | Monkey(tamarin) lymphocyte cell line producing EBV. | Other Name | |
| Animal | cotten-top tamarin | Strain | |
| Genus | Saguinas | Species | oedipus |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | hemo-lymphocytic | |
| Case history | leukocytes | Metastasis | |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | EBV transformed lymphocyte cell line. Cells are releasing EBV (herpesvirus 4) virus. |
| Classify | transformed | Established by | Caputo,et.al. |
| Registered by | Regulation for Distribution | The B95-8 cell line must be handled in the BSL-2 labotory since EBV can infect primates. | |
| Comment | Marmoset, used to be described in the animal name section, was a mistake and corrected to the name, cotton-top tamarin with the scientific name. This cell line is used as a source of EBV. | Year | 1993 |
| Medium | RPMI1640 medium with 10% heat inactivated fetal calf serum. | Methods for Passages | Simple dilution. |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | blood |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:11778691 | Molecular cytogenetic characterization of the EBV-producing cell line B95-8 (Saguinus oedipus, Platyrrhini) by chromosome sorting and painting. Müller S,Neusser M,O'Brien PC,Wienberg J Chromosome Res. 2001;9(8):689-93 |
| Pubmed id:6086497 | Presence of retrovirus in the B95-8 Epstein-Barr virus-producing cell line from different sources. Tumilowicz JJ,Gallick GE,East JL,Pathak S,Trentin JJ,Arlinghaus RB In Vitro. 1984 Jun;20(6):486-92 |
| Pubmed id:4357683 | Comparison of the yield of infectious virus from clones of human and simian lymphoblastoid lines transformed by Epstein-Barr virus. Miller G,Lipman M J Exp Med. 1973 Dec 1;138(6):1398-412 |
| Pubmed id:4346033 | Release of infectious Epstein-Barr virus by transformed marmoset leukocytes. Miller G,Lipman M Proc Natl Acad Sci U S A. 1973 Jan;70(1):190-4 |
| Pubmed id:4333982 | Epstein-Barr virus: transformation, cytopathic changes, and viral antigens in squirrel monkey and marmoset leukocytes. Miller G,Shope T,Lisco H,Stitt D,Lipman M Proc Natl Acad Sci U S A. 1972 Feb;69(2):383-7 |
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LOT Information
| Cell No. | JCRB9123 | Cell Name | B95-8 |
|---|---|---|---|
| LOT No. | 07042014 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO catalog no.11875) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) | Temperature | 37 C |
| Cell Density at Seeding | Methods for Passages | Dilution | |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 5.99x10^5 |
| Viability at cell freezing (%) | 98.5 | Antibiotics Used | free |
| Passage Number | p5* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB9123 | Cell Name | B95-8 |
|---|---|---|---|
| LOT No. | 072795 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% heat inactivated fetal bovine serum (ICN flow 1010183) | Temperature | 37 C |
| Cell Density at Seeding | 3.9x10^5 cells/ml | Methods for Passages | dilution |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.7x10^6 |
| Viability at cell freezing (%) | 95.9 | Antibiotics Used | free |
| Passage Number | P3* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | under examination |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB9123 | Cell Name | B95-8 |
|---|---|---|---|
| LOT No. | 012593 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% heat inactivated fetal bovine serum | Temperature | 37 C |
| Cell Density at Seeding | 2.8x10^5/m | Methods for Passages | simple dilution |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.3x10^6 |
| Viability at cell freezing (%) | 91.1 | Antibiotics Used | free |
| Passage Number | P2* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |