JCRB0834 NUGC-4

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0834 Cell Name   NUGC-4
Profile   adenocarcinoma, poorly differentiated, signet ring cell carcinoma Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   35
Identity   available Tissue for Primary Cancer   stomach
Case history   poorly differ. adenocarcinoma Metastasis   Yes
Tissue Metastasized   lymph node Genetics   
Life Span   infinite Crisis PDL   
Morphology   spherical Character   signet ring cell carcinoma
Classify   tumor Established by   Akiyama,S.
Registered by   Akiyama,M. Regulation for Distribution   
Comment    Year   1993
Medium   RPMI1640 medium with 10% fetal calf serum. Methods for Passages   Simple dilution.
Cell Number on Passage   2.0x10^5 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   gastric lymph node
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3082
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 1298357
Reference
Pubmed id:2121682Alloimmunization with cultured human stomach cancer cell lines and the establishment of human-human hybridomas producing monoclonal antibodies.
Koyama K,Akiyama K,Kawahara H,Egashira A,Murakami H
Jpn J Cancer Res. 1990 Oct;81(10):967-70
Pubmed id:3172586Characteristics of three human gastric cancer cell lines, NU-GC-2, NU-GC-3 and NU-GC-4.
Akiyama S,Amo H,Watanabe T,Matsuyama M,Sakamoto J,Imaizumi M,Ichihashi H,Kondo T,Takagi H
Jpn J Surg. 1988 Jul;18(4):438-46

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LOT Information

Cell No.   JCRB0834 Cell Name   
LOT No.   11272012 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   2.2 - 2.7 x 10^5 cells/mL Methods for Passages   Essentially pipetting. In case adherent cells still exist, use 0.25% trypsin and 0.02% EDTA to detach the cells.
Doubling Time    Cell Number in Vial (cells/1ml)   1.0 x 10^6
Viability at cell freezing (%)   92.0 Antibiotics Used   free
Passage Number   P20 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0834 Cell Name   NUGC-4
LOT No.   10012010 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   1.2 - 1.4 x 10^5 cells/ml Methods for Passages   Subcultivate floating cells as well as attached cells after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   36 hrs Cell Number in Vial (cells/1ml)   2.2 x 10^6
Viability at cell freezing (%)   95.4 Antibiotics Used   free
Passage Number   P20 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   94% Additional information   
Cell No.   JCRB0834 Cell Name   
LOT No.   07042008 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% heat-inactivated fetal bovine serum (GIBCO Cat.# 10099, lot 484375) Temperature   37 C
Cell Density at Seeding   approx. 1.5 - 2 x 10^5 cells/ml Methods for Passages   Subcultivate floating cells as well as attached cells after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   58 hrs Cell Number in Vial (cells/1ml)   2.8 x 10^6
Viability at cell freezing (%)   90.7 Antibiotics Used   free
Passage Number   P21 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0834 Cell Name   
LOT No.   01212004 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   1.5 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. NUGC-4 was reported to subcultivate by simple dilution, but the cells were hardly detached by pipetting.
Doubling Time   D.T. = ca. 2 days Cell Number in Vial (cells/1ml)   2.5 x 10^6
Viability at cell freezing (%)   84.1 Antibiotics Used   free
Passage Number   P20 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0834 Cell Name   NUGC-4
LOT No.   031197 Lot Specification   distribution
Medium   RPMI1640 medium with 10% heat inactivated fetal calf serum (Mitsubishi PVF01). Temperature   37 C
Cell Density at Seeding   2.1x10^5 cells/ml Methods for Passages   simple dilution
Doubling Time   26.9 hrs Cell Number in Vial (cells/1ml)   2.8x10^6
Viability at cell freezing (%)   93.0 Antibiotics Used   free
Passage Number   P17 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0834 Cell Name   
LOT No.   090495 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal calf serum Temperature   37 C
Cell Density at Seeding    Methods for Passages   simple dilution
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0834 Cell Name   NUGC-4
LOT No.   01152016 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091). Temperature   37 C
Cell Density at Seeding   1.7 - 1.9 x 10^5 cells/mL Methods for Passages   Essentially cells were detached by pipetting. In case adherent cells still exist, use 0.25% trypsin and 0.02% EDTA to detach the cells.
Doubling Time   approx. 40 hrs. Cell Number in Vial (cells/1ml)   4.7 x 10^6
Viability at cell freezing (%)   90 Antibiotics Used   free
Passage Number   P20 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   90 Additional information   
Cell No.   JCRB0834 Cell Name   NUGC-4
LOT No.   01262018 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   1.3 - 1.4 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture).
Doubling Time   approx. 36 hrs. Cell Number in Vial (cells/1ml)   9.5 x 10^5
Viability at cell freezing (%)   76.6 Antibiotics Used   free
Passage Number   P21 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   76.6 Additional information   
Cell No.   JCRB0834 Cell Name   NUGC-4
LOT No.   04152019 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.3 - 2.2 x 10^5 Methods for Passages   Simple dilution. Essentially cells were detached by pipetting. In case adherent cells still exist, use 0.25% trypsin and 0.02% EDTA to detach the cells.
Doubling Time   approx. 36 hrs. Cell Number in Vial (cells/1ml)   3.9 x 10^6
Viability at cell freezing (%)   92.2 Antibiotics Used   free
Passage Number   P20 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   N/Y Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   92.3 Additional information   
Cell No.   JCRB0834 Cell Name   NUGC-4
LOT No.   02042021 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   2.1 - 2.6 x 10^5 cells/mL Methods for Passages   Simple dilution. Essentially cells were detached by pipetting. In case adherent cells still exist, use 0.25% trypsin and 0.02% EDTA to detach the cells.
Doubling Time   approx. 42 hrs. Cell Number in Vial (cells/1ml)   5.2 x 10^6
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   P20 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   90 Additional information   
Cell No.   JCRB0834 Cell Name   NUGC-4
LOT No.   03142022 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Biowest Cat. # S1820-500) Temperature   37 C
Cell Density at Seeding   1.4 - 2.2 x 10^5 cells/mL Methods for Passages    Simple dilution. Essentially cells were detached by pipetting. In case adherent cells still exist, use 0.25% trypsin and 0.02% EDTA to detach the cells.
Doubling Time   36 - 52 hrs. Cell Number in Vial (cells/1ml)   4.1 x 10^6
Viability at cell freezing (%)   96.6 Antibiotics Used   free
Passage Number   P20 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Y/N Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   94.9 Additional information   
Cell No.   JCRB0834 Cell Name   NUGC-4
LOT No.   02292024 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   1.4 - 4.1 x 10^5 cells/mL Methods for Passages   Simple dilution. Essentially cells were detached by pipetting. In case adherent cells still exist, use 0.25% trypsin and 0.02% EDTA to detach the cells.
Doubling Time   approx. 35 hrs. Cell Number in Vial (cells/1ml)   2.4 x 10^6
Viability at cell freezing (%)   92.9 Antibiotics Used   free
Passage Number   P20 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Y/N Exoteric Gene   
Medium for Freezing   5% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   90.4 Additional information   
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