JCRB0432 KMS-6

Cell information

Important Notice(s)

JCRB0432 KMS-6: growth problem

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0432 Cell Name   KMS-6
Profile   fibroblast, normal diploid Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   9-week-old
Identity   available Tissue for Primary Cancer   embryo
Case history   normal fibroblast. Metastasis   
Tissue Metastasized    Genetics   Normal diploid cell
Life Span   finite Crisis PDL   
Morphology   fibroblast-like Character   
Classify   normal Established by   Namba,M., et.al.
Registered by   Namba,M. Regulation for Distribution   No commercial purpose.
Comment   Change all medium as soon as received. Year   1994
Medium   Eagle's minimal essential medium with 10% fetal bovine serum. Methods for Passages   Cells treated with 0.2% trypsin in PBS(-). Rubber policeman will not be recommended.
Cell Number on Passage   Split ratio=1/2. Race   
CO2 Conc.   5 % Tissue Sampling   embryo
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2997
Reference
Pubmed id:3972471Neoplastic transformation of human diploid fibroblasts (KMST-6) by treatment with 60Co gamma rays.
Namba M,Nishitani K,Hyodoh F,Fukushima F,Kimoto T
Int J Cancer. 1985 Feb 15;35(2):275-80

Images



LOT Information

Cell No.   JCRB0432 Cell Name   KMS-6
LOT No.   051598 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal calf serum (Mitsubishi PVF01). Temperature   37 C
Cell Density at Seeding   1.2x10^4 cells/sq.cm. Methods for Passages   Cells are harvested with 0.25% trypsin and 0.02% EDTA for 5 min. at room temperature.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   94.0 Antibiotics Used   MC210 added to the culture to remove mycoplasmas.
Passage Number   P21 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0432 Cell Name   KMS-6
LOT No.   042198 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal calf serum (Mitsubishi PVF01). Temperature   37 C
Cell Density at Seeding   1.2 x 10^4 cells/sq.cm. Methods for Passages   Cells are harvested with 0.25% trypsin and 0.02% EDTA treatment (5 min. at room temperature).
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.7x10^6
Viability at cell freezing (%)   89.0 Antibiotics Used   free
Passage Number   P17 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,12
D13S317:10,11
D7S820:9
D16S539:9,11
VWA:14,18
TH01:9
AM:X
TPOX:11,12
CSF1PO:9,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0432 Cell Name   KMS-6
LOT No.   042098 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal calf serum (Mitsubishi, PVF01). Temperature   37 C
Cell Density at Seeding   1.2x10^4 cells/sq.cm. Methods for Passages   Cells are harvested with 0.25% trypsin and 0.02% EDTA for 5 min. at room temperature.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.92x10^6
Viability at cell freezing (%)   89.0 Antibiotics Used   MC210 treated to remove mycoplasmas.
Passage Number   P17 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
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