JCRB0433 KMST-6

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0433 Cell Name   KMST-6
Profile   fibroblast, immortalized by 60Co irradiation, derivative of KMS-6 Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   9-week-old
Identity   available Tissue for Primary Cancer   embryo
Case history   normal fibroblasts immortalized by 60Co irradiation. Metastasis   
Tissue Metastasized    Genetics   
Life Span   finite Crisis PDL   
Morphology   fibroblast-like Character   indefinite growth and non-tumorigenic
Classify   transformed Established by   Namba,M., et.al.
Registered by   Namba,M. Regulation for Distribution   No commercial purpose.
Comment    Year   1994
Medium   Eagle's minimal essentila medium with 10% fetal bovine serum. Methods for Passages   Cells treated with 0.2% trypsin in PBS(-).
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   embryo
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2998
Reference
Pubmed id:1537630Malignant transformation of human fibroblasts previously immortalized with 60Co gamma rays.
Mihara K,Bai L,Kano Y,Miyazaki M,Namba M
Int J Cancer. 1992 Feb 20;50(4):639-43
Pubmed id:3287150Multi-step neoplastic transformation of normal human fibroblasts by Co-60 gamma rays and Ha-ras oncogenes.
Namba M,Nishitani K,Fukushima F,Kimoto T,Yuasa Y
Mutat Res. 1988 Jun;199(2):415-23
Pubmed id:3140712Multistep carcinogenesis of normal human fibroblasts. Human fibroblasts immortalized by repeated treatment with Co-60 gamma rays were transformed into tumorigenic cells with Ha-ras oncogenes.
Namba M,Nishitani K,Fukushima F,Kimoto T
Anticancer Res. 1988 Sep-Oct;8(5A):947-58
Pubmed id:3972471Neoplastic transformation of human diploid fibroblasts (KMST-6) by treatment with 60Co gamma rays.
Namba M,Nishitani K,Hyodoh F,Fukushima F,Kimoto T
Int J Cancer. 1985 Feb 15;35(2):275-80

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LOT Information

Cell No.   JCRB0433 Cell Name   KMST-6
LOT No.   05292002 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal calf serum (Intergen RB52405). Temperature   37 C
Cell Density at Seeding   2.5 x 10^4 cells/sq.cm. Methods for Passages   Cells were harvested after treatment with 0.2% trypsin. Subculture once a week and medium change once a week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.8 x 10^6
Viability at cell freezing (%)   95.2 Antibiotics Used   MC210 treatment done only for this culture.
Passage Number   P120 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), LD and NP examined. Human not a HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,12
D13S317:10,11
D7S820:9
D16S539:11
VWA:14,18
TH01:9
AM:X
TPOX:11,12
CSF1PO:9,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium containing 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0433 Cell Name   KMST-6
LOT No.   deposit Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum. Temperature   37 C
Cell Density at Seeding   split ratio=1/2 - 1/4. Methods for Passages   Treat cells with 0.2% trypsin in Ca & Mg free phosphate buffered saline.
Doubling Time   60 hrs Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   100ug/ml kanamycin
Passage Number    PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD B type, LDH human type.
Chromosome Mode   53 Chromosome Information   heteroploid
Surface Antigen   NT DNA Profile (STR)   D5S818:10,12
D13S317:10,11
D7S820:9
D16S539:11
VWA:14,18
TH01:9
AM:X
TPOX:11,12
CSF1PO:9,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0433 Cell Name   KMST-6
LOT No.   12242014 Lot Specification   distribution
Medium   Eagle's minimum essentila medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091). Temperature   37 C
Cell Density at Seeding   3.0 - 10 x 10^4 cells/mL Methods for Passages   cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 26 hrs. Cell Number in Vial (cells/1ml)   5.4 x 10^6
Viability at cell freezing (%)   89.8 Antibiotics Used   free
Passage Number   P123 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   89.8 Additional information   
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