JCRB0744 ECV304

Cell information

Important Notice(s)

Cross-contamination between ECV304 and T24

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0744 Cell Name   ECV304
Profile   Stop providing for the issue of quality control. (Please contact us for more information) Originally this cell line was established as human endothelium cells from umbilical cord but later the cell line was identified to be an EJ1/T24 by STR-PCR analysis Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   0-years-old
Identity   available Tissue for Primary Cancer   vascular system
Case history   normal Metastasis   No
Tissue Metastasized    Genetics   human immortalized endothelium cell
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   human immortalized endothelium cell line from umbilical cord. But JCRB identified the cell line is derived from cross contamination with famous EJ1/T24 cells by STR-PCR analysis.
Classify   transformed Established by   Takahashi,K.
Registered by   Umeda,M. Regulation for Distribution   Refer Ref.{3581} when publish paper using the cell line ECV304.
Comment   Dr. Drexler of the DSM informed to us that the ECV304 was crosscontaminated with EJ1/T24 at the end of 1998. Following his suggestion the JCRB examined by STR-PCR method and confirmed the crosscontamination. Year   1991
Medium   M199 medium with 10% fetal bovine serum. Methods for Passages   Cells are hrvested after treatment with 0.125% trypsin and 0.02% EDTA.
Cell Number on Passage   7.3 x 10^3 cells/sq.cm. Race   
CO2 Conc.   5 % Tissue Sampling   umbilical cord
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2029
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 724812
Reference
Pubmed id:1378828Rare spontaneously transformed human endothelial cell line provides useful research tool.
Takahasi K,Sawasaki Y
In Vitro Cell Dev Biol. 1992 Jun;28A(6):380-2
Pubmed id:1690702Spontaneous transformation and immortalization of human endothelial cells.
Takahashi K,Sawasaki Y,Hata J,Mukai K,Goto T
In Vitro Cell Dev Biol. 1990 Mar;26(3 Pt 1):265-74

Images
CLOSE