JCRB0745 T-23 clone 8 Cell information
Cell type:general cells
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JCRB No.
JCRB0745
Cell Name
T-23 clone 8
Profile
normal, applicable for the mutation assay system in vitro
Other Name
T-238
Animal
tupaia
Strain
Genus
Tupaia
Species
belangeri
Sex
M
Age
young
Identity
Tissue for Primary Cancer
lung
Case history
normal
Metastasis
No
Tissue Metastasized
Genetics
normal
Life Span
finite
Crisis PDL
Morphology
epithelial-like
Character
Applicable for the mutation assay system in vitro.
Classify
normal
Established by
Inui,N. & Taketomi,M.
Registered by
Umeda,M.
Regulation for Distribution
Not applied.
Comment
Year
1991
Medium
Methods for Passages
Cell Number on Passage
Race
CO2 Conc.
5 %
Tissue Sampling
lung
Tissue Type
Detection of virus genome fragment by Real-time PCR
Detected DNA Virus
not tested
Detected RNA Virus
not tested
Reference
Pubmed id:3331674 Establishment of lung fibroblastic cell lines from a non-human primate Tupaia belangeri and their use in a forward gene mutation assay at the hypoxanthine-guanine phosphoribosyl transferase locus. Taketomi M,Nishi Y,Ohkawa Y,Inui N Mutagenesis. 1986 Sep;1(5):359-65
LOT Information
Cell No.
JCRB0745
Cell Name
LOT No.
02142013
Lot Specification
distribution
Medium
Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS, GIBCO Cat. # 10099)
Temperature
37 C
Cell Density at Seeding
0.8 - 1.5 x 10^5 cells/mL
Methods for Passages
Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time
22 - 37 hrs.
Cell Number in Vial (cells/1ml)
2.0 x 10^6
Viability at cell freezing (%)
96.5
Antibiotics Used
free
Passage Number
Unknown (7 at bank)
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Yes
Exoteric Gene
Medium for Freezing
10% DMSO, 20% FBS - DMEM
CO2 Conc.
5%
Viability immediately after thawing (%)
Additional information