JCRB0119 YG10003
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0119 | Cell Name | YG10003 |
|---|---|---|---|
| Profile | salmonella O-acetyltransferase gene introduced CHL cells | Other Name | |
| Animal | hamster, Chinese | Strain | Chinese |
| Genus | Cricetulus | Species | griseus |
| Sex | F | Age | |
| Identity | Tissue for Primary Cancer | lung | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | salmonella O-acetyltransferase gene introduced | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | G418-r(500ug/ml), pMAMneo cotransfected |
| Classify | artificial | Established by | Watanabe,M. et.al. |
| Registered by | Nohmi,T. | Regulation for Distribution | not regulated |
| Comment | This cells are comming from NIHS0160 after quality controls. | Year | 1993 |
| Medium | Eagle's minimal essential medium with 10% calf serum and 500 ug/ml G-418. | Methods for Passages | Cells are treated with 0.25% trypsin and 0.02% EDTA. G-418 is required to maitain extra DNA fragment. Recombinant cell. |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | lung |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:8137280 | New sublines of Chinese hamster CHL stably expressing human NAT1 or NAT2 N-acetyltransferases or Salmonella typhimurium O-acetyltransferase: comparison of the sensitivities to nitroarenes and aromatic amines using the in vitro micronucleus test. Watanabe M,Matsuoka A,Yamazaki N,Hayashi M,Deguchi T,Nohmi T,Sofuni T Cancer Res. 1994 Apr 1;54(7):1672-7 |
| Pubmed id:876270 | Chromosome tests with 134 compounds on Chinese hamster cells in vitro--a screening for chemical carcinogens. Ishidate M Jr,Odashima S Mutat Res. 1977 Jul;48(3-4):337-53 |
| Pubmed id:5452405 | A new cell line derived from newborn Chinese hamster lung tissue. Koyama H,Utakoji T,Ono T Gan. 1970 Apr;61(2):161-7 |
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LOT Information
| Cell No. | JCRB0119 | Cell Name | YG10003 |
|---|---|---|---|
| LOT No. | 041795 | Lot Specification | distribution |
| Medium | Eagle's minimal essential medium with 10% calf serum (ICN Flow,1059023) | Temperature | 37 C |
| Cell Density at Seeding | 1.6x10^6 cells/10cm dish | Methods for Passages | Cells are treated with 0.25% trypsin and 0.02% EDTA and diluted. |
| Doubling Time | 20.3 hrs | Cell Number in Vial (cells/1ml) | 5.7x10^6 |
| Viability at cell freezing (%) | 98.2 | Antibiotics Used | free |
| Passage Number | P19* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | G6PD, LDH, NP (hamster type) |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |