JCRB0119 YG10003

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0119 Cell Name   YG10003
Profile   salmonella O-acetyltransferase gene introduced CHL cells Other Name   
Animal   hamster, Chinese Strain   Chinese
Genus   Cricetulus Species   griseus
Sex   F Age   
Identity    Tissue for Primary Cancer   lung
Case history    Metastasis   
Tissue Metastasized    Genetics   salmonella O-acetyltransferase gene introduced
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   G418-r(500ug/ml), pMAMneo cotransfected
Classify   artificial Established by   Watanabe,M. et.al.
Registered by   Nohmi,T. Regulation for Distribution   not regulated
Comment   This cells are comming from NIHS0160 after quality controls. Year   1993
Medium   Eagle's minimal essential medium with 10% calf serum and 500 ug/ml G-418. Methods for Passages   Cells are treated with 0.25% trypsin and 0.02% EDTA. G-418 is required to maitain extra DNA fragment. Recombinant cell.
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_8557
Pubmed id:8137280New sublines of Chinese hamster CHL stably expressing human NAT1 or NAT2 N-acetyltransferases or Salmonella typhimurium O-acetyltransferase: comparison of the sensitivities to nitroarenes and aromatic amines using the in vitro micronucleus test.
Watanabe M,Matsuoka A,Yamazaki N,Hayashi M,Deguchi T,Nohmi T,Sofuni T
Cancer Res. 1994 Apr 1;54(7):1672-7
Pubmed id:876270Chromosome tests with 134 compounds on Chinese hamster cells in vitro--a screening for chemical carcinogens.
Ishidate M Jr,Odashima S
Mutat Res. 1977 Jul;48(3-4):337-53
Pubmed id:5452405A new cell line derived from newborn Chinese hamster lung tissue.
Koyama H,Utakoji T,Ono T
Gan. 1970 Apr;61(2):161-7


LOT Information

Cell No.   JCRB0119 Cell Name   YG10003
LOT No.   041795 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% calf serum (ICN Flow,1059023) Temperature   37 C
Cell Density at Seeding   1.6x10^6 cells/10cm dish Methods for Passages   Cells are treated with 0.25% trypsin and 0.02% EDTA and diluted.
Doubling Time   20.3 hrs Cell Number in Vial (cells/1ml)   5.7x10^6
Viability at cell freezing (%)   98.2 Antibiotics Used   free
Passage Number   P19* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, LDH, NP (hamster type)
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information