JCRB9125 PG-4(S+L-)
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB9125 | Cell Name | PG-4(S+L-) |
|---|---|---|---|
| Profile | brain derived cell line transformed with Moloney murine sarcoma virus, support type C retroviruses | Other Name | PG-4 |
| Animal | cat | Strain | |
| Genus | Felis | Species | |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | neural | |
| Case history | normal | Metastasis | |
| Tissue Metastasized | Genetics | Transformed with Moloney murine sarcoma virus,MMSV | |
| Life Span | infinite | Crisis PDL | |
| Morphology | glial/astrocyte | Character | Support Type C retroviruses. |
| Classify | transformed | Established by | |
| Registered by | Dunn,K.J. | Regulation for Distribution | ATCC |
| Comment | Year | 1994 | |
| Medium | McCoy's 5a medium with 10% heat-inactivated fetal bovine serum. | Methods for Passages | |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | brain |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:2983093 | Isolation from cats of an endogenous type C virus with a novel envelope glycoprotein. Haapala DK,Robey WG,Oroszlan SD,Tsai WP J Virol. 1985 Mar;53(3):827-33 |
| Pubmed id:6959413 | Normal DBA/2 mouse cells synthesize a glycoprotein which interferes with MCF virus infection. Bassin RH,Ruscetti S,Ali I,Haapala DK,Rein A Virology. 1982 Nov;123(1):139-51 |
| Images |
|---|
      |
LOT Information
| Cell No. | JCRB9125 | Cell Name | PG-4(S+L-) |
|---|---|---|---|
| LOT No. | 08042005 | Lot Specification | distribution |
| Medium | McCoy's 5a medium with 10% heat-inactivated fetal bovine serum (FBS lot; GIBCO 4218333S) | Temperature | 37 C |
| Cell Density at Seeding | 7 - 9 x 10^4 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA |
| Doubling Time | approx. 16 hrs | Cell Number in Vial (cells/1ml) | 3.3 x 10^6 |
| Viability at cell freezing (%) | 98.0 | Antibiotics Used | free |
| Passage Number | P26 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as cat by NP, G6PD, AST. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - McCoy's 5a | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information | Cell name is labeled as PG-4. |
| Cell No. | JCRB9125 | Cell Name | PG-4(S+L-) |
|---|---|---|---|
| LOT No. | 102194 | Lot Specification | distribution |
| Medium | McCoy's 5a medium with 10% heat inactivated fetal bovine serum (Hyclone 11151031) | Temperature | 37 C |
| Cell Density at Seeding | 1.6x10^6cells/10cm dish | Methods for Passages | Treat cells twice with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.25x10^7 |
| Viability at cell freezing (%) | 84.0 | Antibiotics Used | free |
| Passage Number | P24 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | G6PD,LDH,NP |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information | Labelled as PG-4 on vials. |