JCRB9126 BS-C-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9126 Cell Name   BS-C-1
Profile   Kidney cell line of the african green monkey. Other Name   
Animal   monkey, African green Strain   
Genus    Species   
Sex    Age   
Identity    Tissue for Primary Cancer   kidney
Case history    Metastasis   
Tissue Metastasized    Genetics   kidney
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   This cell line is a suitable host for transfection experiments of SV40 vectors.
Classify   normal Established by   Hopps,H.E. et al.
Registered by   JCRB Regulation for Distribution   
Comment   Prepared from CCL 26. Year   1997
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% calf serum Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin.
Cell Number on Passage   split ratio=1/2 - 1/4 Race   
CO2 Conc.   5 % Tissue Sampling   kidney
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0607
Reference
Pubmed id:3224379A novel 53 kDa actin binding protein from porcine brain--further biochemical and immunological characterization.
Maekawa S,Ohta K,Sakai H
Cell Struct Funct. 1988 Oct;13(5):373-85
Pubmed id:3465720Purification and properties of epithelial growth inhibitor (EGI) from human platelets: its separation from type beta transforming growth factor (TGF-beta).
Huang W,Kimura T,Mashima K,Miyazaki K,Masaki H,Yamashita J,Horio T
J Biochem. 1986 Sep;100(3):687-96
Pubmed id:6304690Rearrangement and mutagenesis of a shuttle vector plasmid after passage in mammalian cells.
Razzaque A,Mizusawa H,Seidman MM
Proc Natl Acad Sci U S A. 1983 May;80(10):3010-4
Pubmed id:4203458A comparison of three in vivo assays for cell tumorigenicity.
Petricciani JC,Wallace RE,McCoy DW
Cancer Res. 1974 Jan;34(1):105-8
Pubmed id:4350227A temperature-sensitive mutant of simian virus 40 affecting transforming ability.
Kimura G,Dulbecco R
Virology. 1973 Apr;52(2):529-34
Pubmed id:14215895THE SENSITIVITY OF GRIVET MONKEY KIDNEY CELL LINE BS-C-1 FOR PROPAGATION AND ISOLATION OF CERTAIN HUMAN VIRUSES.
SCHMIDT NJ,LENNETTE EH,SHON CW,DENNIS J
Am J Public Health Nations Health. 1964 Sep;54():1522-30
Pubmed id:14071033BIOLOGIC CHARACTERISTICS OF A CONTINUOUS KIDNEY CELL LINE DERIVED FROM THE AFRICAN GREEN MONKEY.
HOPPS HE,BERNHEIM BC,NISALAK A,TJIO JH,SMADEL JE
J Immunol. 1963 Sep;91():416-24

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LOT Information

Cell No.   JCRB9126 Cell Name   BS-C-1
LOT No.   032797 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 0.1mM non-essential amino acids with 10% fetal calf serum/PVF1 Temperature   37 C
Cell Density at Seeding   1.5x10^4 cells/sq.cm. Methods for Passages   Cells harvested with 0.25% trypsin and 0.02% EDTA for 5 minutes at room temperature.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.8x10^6
Viability at cell freezing (%)   91.0 Antibiotics Used   free
Passage Number   P49 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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