JCRB0435 JHH-4

Cell information

Important Notice(s)

On the agreement for distribution of JHH series cell lines, NOZ and OZ

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0435 Cell Name   JHH-4
Profile   Human hepatocellular carcinoma cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   51
Identity   available Tissue for Primary Cancer   liver, gallbladder
Case history   hepatocellular carcinoma Metastasis   
Tissue Metastasized    Genetics   HBV integration is not observed.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Alpha-phetoprotein and albumin-producing cell line. HBs antigen-negative. HCV was not detected by an RT-PCR method tested in the HSRRB.
Classify   tumor Established by   Hasumura,S. et al.
Registered by   Nagamori,S. Regulation for Distribution   Restricted: Cell's developer's approval is required
Comment   Cell Bank will handle to get the approval but the depositor may contact to the requested person directly. Year   1988
Medium   Eagle's minimal essential medium with 10% fetal calf serum. Methods for Passages   Cells are harvested after the treatment with 0.25% trypsin and 0.02% EDTA at room temp. for 5 min.
Cell Number on Passage   1-2x10^4 cells/sq.cm. Race   Japanese
CO2 Conc.   5 % Tissue Sampling   liver
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2787
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 1240158
Reference
Pubmed id:2856444[Establishment and characterization of a human hepatocellular carcinoma cell line JHH-4].
Hasumura S,Sujino H,Nagamori S,Kameda H
Hum Cell. 1988 Mar;1(1):98-100


Research results by users. Click！
Pubmed id:31324698 The 2-Hydroxyiminostilbene Metabolite of Carbamazepine or the Supernatant from Incubation of Hepatocytes with Carbamazepine Activates Inflammasomes: Implications for Carbamazepine-Induced Hypersensitivity Reactions.
Kato R,Ijiri Y,Hayashi T,Uetrecht J
Drug Metab Dispos. 2019 Oct;47(10):1093-1096
Pubmed id:29087505 Editor's Highlight: An Impaired Immune Tolerance Animal Model Distinguishes the Potential of Troglitazone/Pioglitazone and Tolcapone/Entacapone to Cause IDILI.
Mak A,Kato R,Weston K,Hayes A,Uetrecht J
Toxicol Sci. 2018 Feb 1;161(2):412-420
Pubmed id:28081667 Contributions of caspase-8 and -9 to liver injury from CYP2E1-produced metabolites of halogenated hydrocarbons.
Ijiri Y,Kato R,Sadamatsu M,Takano M,Yasuda Y,Tanaka F,Oishi C,Imano H,Okada Y,Tanaka K,Hayashi T
Xenobiotica. 2018 Jan;48(1):60-72
Pubmed id:28525267 Supernatant from Hepatocyte Cultures with Drugs That Cause Idiosyncratic Liver Injury Activates Macrophage Inflammasomes.
Kato R,Uetrecht J
Chem Res Toxicol. 2017 Jun 19;30(6):1327-1332
Pubmed id:27183710 Effect of hypoxia on UDP-glucuronosyl transferase mRNA expression in human hepatocarcinoma functional liver celL4 cell line.
Kato R,Matsura A,Kamiya R,Oishi C,Kagawa Y,Tanaka F,Matsumoto N,Ijiri Y,Hayashi T
Pharmazie. 2016 Mar;71(3):152-3
Pubmed id:24599999 Novel permissive cell lines for complete propagation of hepatitis C virus.
Shiokawa M,Fukuhara T,Ono C,Yamamoto S,Okamoto T,Watanabe N,Wakita T,Matsuura Y
J Virol. 2014 May;88(10):5578-94
Pubmed id:20044606 Infrequent amplification of JUN in hepatocellular carcinoma.
Endo M,Yasui K,Nakajima T,Gen Y,Tsuji K,Dohi O,Zen K,Mitsuyoshi H,Minami M,Itoh Y,Taniwaki M,Tanaka S,Arii S,Okanoue T,Yoshikawa T
Anticancer Res. 2009 Dec;29(12):4989-94
Pubmed id:18248513 Chemosensitivity determinants of irinotecan hydrochloride in hepatocellular carcinoma cell lines.
Takahata T,Ookawa K,Suto K,Tanaka M,Yano H,Nakashima O,Kojiro M,Tamura Y,Tateishi T,Sakata Y,Fukuda S
Basic Clin Pharmacol Toxicol. 2008 Apr;102(4):399-407
Pubmed id:17549407 Activation of a system A amino acid transporter, ATA1/SLC38A1, in human hepatocellular carcinoma and preneoplastic liver tissues.
Kondoh N,Imazeki N,Arai M,Hada A,Hatsuse K,Matsuo H,Matsubara O,Ohkura S,Yamamoto M
Int J Oncol. 2007 Jul;31(1):81-7
Pubmed id:16041541 Interferon-alpha/beta upregulate IL-15 expression in vitro and in vivo: analysis in human hepatocellular carcinoma cell lines and in chronic hepatitis C patients during interferon-alpha/beta treatment.
Yamaji K,Nabeshima S,Murata M,Chong Y,Furusyo N,Ikematsu H,Hayashi J
Cancer Immunol Immunother. 2006 Apr;55(4):394-403
Pubmed id:16522372 A comparison of the antitumor effects of interferon-alpha and beta on human hepatocellular carcinoma cell lines.
Murata M,Nabeshima S,Kikuchi K,Yamaji K,Furusyo N,Hayashi J
Cytokine. 2006 Feb 7;33(3):121-8
Pubmed id:15349899 Proteomic signature corresponding to alpha fetoprotein expression in liver cancer cells.
Yokoo H,Kondo T,Fujii K,Yamada T,Todo S,Hirohashi S
Hepatology. 2004 Sep;40(3):609-17
Pubmed id:10766424 Involvement of insulin-like growth factor binding protein-3 in the retinoic acid receptor-alpha-mediated inhibition of hepatocellular carcinoma cell proliferation.
Murakami K,Matsuura T,Hasumura S,Nagamori S,Yamada Y,Saiki I
Cancer Lett. 2000 Apr 3;151(1):63-70
Pubmed id:9446792 Cyclin E overexpression responsible for growth of human hepatic tumors with p21WAF1/CIP1/SDI1.
Tsuji T,Miyazaki M,Fushimi K,Mihara K,Inoue Y,Ohashi R,Ohtsubo M,Hamazaki K,Furusako S,Namba M
Biochem Biophys Res Commun. 1998 Jan 14;242(2):317-21
Pubmed id:8835345 Persistence of hepatitis C virus RNA in established human hepatocellular carcinoma cell lines.
Tsuboi S,Nagamori S,Miyazaki M,Mihara K,Fukaya K,Teruya K,Kosaka T,Tsuji T,Namba M
J Med Virol. 1996 Feb;48(2):133-40
Pubmed id:1315167 Induction of allogeneic tumour- and lymphokine-activated lymphocytes against hepatocellular carcinoma.
Yasumura S,Higuchi K,Hioki O,Okada K,Tsukishiro T,Tsuchida T,Miyagiwa M,Nambu S,Yasuyama T,Inoue K
J Gastroenterol Hepatol. 1992 Mar-Apr;7(2):136-41
Pubmed id:1651985 Preferential expression of the large hepatitis B virus surface antigen gene by an adenovirus-hepatitis B virus recombinant.
Yuasa T,Kajino K,Saito I,Miyamura T
J Gen Virol. 1991 Aug;72 ( Pt 8)():1927-34
Pubmed id:1701409 Integration of hepatitis B virus DNA into cells of six established human hepatocellular carcinoma cell lines.
Fujise K,Nagamori S,Hasumura S,Homma S,Sujino H,Matsuura T,Shimizu K,Niiya M,Kameda H,Fujita K
Hepatogastroenterology. 1990 Oct;37(5):457-60
Pubmed id:2543327 [Combination therapy of hyperthermia and other methods in liver and bile tract cancers--evaluation of these methods using cancer cell lines in vitro].
Hasumura S,Nagamori S,Fujise K,Homma S,Sujino H,Matsuura T,Shimizu K,Niiya M,Kameda H
Gan To Kagaku Ryoho. 1989 Apr;16(4 Pt 2-3):1905-12
Pubmed id:2856500 [Effects of TNF on human hepatocellular carcinoma cell lines and their modification by hyperthermia].
Hasumura S,Nagamori S,Fujise K,Homma S,Sujino H,Matsuura T,Shimizu K,Niiya M,Kameda H,Satomi N
Hum Cell. 1988 Jun;1(2):238-44
Pubmed id:2856444 [Establishment and characterization of a human hepatocellular carcinoma cell line JHH-4].
Hasumura S,Sujino H,Nagamori S,Kameda H
Hum Cell. 1988 Mar;1(1):98-100
Pubmed id:3428672 An epidemic of pseudomembranous colitis: importance of person to person spread.
Nolan NP,Kelly CP,Humphreys JF,Cooney C,O'Connor R,Walsh TN,Weir DG,O'Briain DS
Gut. 1987 Nov;28(11):1467-73
Pubmed id:none THE MORPHOLOGICAL STUDY OF HUMAN CULTURED HEPATOMA CELLS BY A NEW PLASMA POLYMERIZATION REPLICA METHOD AND TEM
Hajime SUJINO
Jikeikai Medical Journal 33(4), 379-400 (1986)
Pubmed id:none ヒト肝癌細胞株に対するTumor necrosis factorの影響
蓮村哲 永森静志 藤瀬清隆 本間定 筋野甫 松浦知和 清水恵一郎 新谷稔 里見信子
医学のあゆみ 143(8), 655-656 (1987)
Pubmed id:none Fluorochromeを用いたchromatin DNAのcolorimetric assay法による単層培養細胞増殖動態の検討
松浦知和 永森静志 藤瀬清隆 蓮村哲 本間定 筋野甫 清水恵一郎 新谷稔 亀田治男
医学のあゆみ 142(13), 957-958 (1987)
Pubmed id:none 樹立ヒト肝癌株細胞中のB型肝炎ウイルスゲノム
藤瀬 清隆, 藤多 和信, 永森 静志, 蓮村 哲, 本間 定, 筋野 甫, 松浦 知和, 清水 恵一郎, 新谷 稔, 大野 典也, 亀田 治男
肝臓 29(5), 697-698 (1988)

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LOT Information

Cell No.   JCRB0435 Cell Name   
LOT No.   04142005 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   5 - 15 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA at room temperature for 5 min.
Doubling Time   approx. 40.5 hrs Cell Number in Vial (cells/1ml)   2.3 x 10^6
Viability at cell freezing (%)   97.1 Antibiotics Used   free
Passage Number   P17 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - Eagle's MEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0435 Cell Name   JHH-4
LOT No.   061897 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal calf serum (Mitsubishi PVF01). Temperature   37 C
Cell Density at Seeding   1-2x10^6 cells/sq.cm. Methods for Passages   Cells are harvested after the treatment with 0.25% trypsin and 0.02% EDTA at room temp. for 5 min.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.0x10^6
Viability at cell freezing (%)   92.0 Antibiotics Used   free
Passage Number   P13 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture mdium with 5% DMSO. CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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Cell No.   JCRB0435 Cell Name   JHH-4
LOT No.   04142014 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal calf serum (FBS; GIBCO Cat. # 10091). Temperature   37 C
Cell Density at Seeding   4.7 - 9.0 x 10^4 cells/mL Methods for Passages   Cells were harvested after the treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   2.9 x 10^6
Viability at cell freezing (%)   96.0 Antibiotics Used   free
Passage Number   P17 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:9,13
D13S317:12
D7S820:10,12
D16S539:12,13
VWA:14,17
TH01:6,9
AM:X
TPOX:8,11
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   96.0 Additional information   Cell Bank will handle to get the approval but the depositor may contact to the requester directly.
Cell No.   JCRB0435 Cell Name   JHH-4
LOT No.   01242022 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Biowest Cat. # S18200-500) Temperature   37 C
Cell Density at Seeding   0.4 - 2.0 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 32 hrs. Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   97 Antibiotics Used   free
Passage Number   P16 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   92 Additional information   
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