JCRB0124 Takigawa
Cell information
Important Notice(s)Growth properties of JCRB0124 Takigawa
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0124 | Cell Name | Takigawa |
|---|---|---|---|
| Profile | gastric carcinoma, proliferates very slowly (D.T.=2 weeks). | Other Name | Takigawa |
| Animal | human | Strain | |
| Genus | Homo | Species | Sapiens |
| Sex | M | Age | 63 |
| Identity | available | Tissue for Primary Cancer | stomach |
| Case history | advanced gastric carcinoma. | Metastasis | |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | islet-like colonies formed | Character | Densly packed ilet-like colonies with small polygonal cells and high AFP production observed. |
| Classify | tumor | Established by | Sekiguchi,M. |
| Registered by | Sekiguchi,M. | Regulation for Distribution | free. |
| Comment | Year | 1997 | |
| Medium | Methods for Passages | ||
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | lymph node |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | not tested | ||||||
| CMV | - | parvoB19 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| EBV | - | HBV | - | ||||||
| HHV6 | - | HTLV-1 | - | ||||||
| HHV7 | - | HTLV-2 | - | ||||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | NT | ||||||
| Notes | |||||||||
| Images |
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LOT Information
| Cell No. | JCRB0124 | Cell Name | |
|---|---|---|---|
| LOT No. | 06282011 | Lot Specification | distribution |
| Medium | 1:1 mixture of Dulbecco's modified Eagle's medium and RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099) | Temperature | 37 C |
| Cell Density at Seeding | 0.3 - 5.0 x 10^5 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Growth was very slow. |
| Doubling Time | Min. 70 hr, Average 10 days. | Cell Number in Vial (cells/1ml) | 2.6 x 10^6 |
| Viability at cell freezing (%) | 80.1 | Antibiotics Used | free |
| Passage Number | P45 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - DMEM/RPMI1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0124 | Cell Name | Takigawa |
|---|---|---|---|
| LOT No. | 073197 | Lot Specification | distribution |
| Medium | 45% Dulbecco's modified Eagle's medium, 45% RPMI1640 medium and 10% fetal calf serum(JRH bio.1B1062) | Temperature | 37 C |
| Cell Density at Seeding | 5.5x10^4 cells/sq.cm. | Methods for Passages | Cells harvested with 0.25% trypsin and 0.02% EDTA for 5 min. at room temperature. Subcult.twice/week |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.3x10^6 |
| Viability at cell freezing (%) | 88.0 | Antibiotics Used | free |
| Passage Number | P276 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Culture medium with 5% DMSO. | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0124 | Cell Name | |
|---|---|---|---|
| LOT No. | 09022004 | Lot Specification | distribution |
| Medium | 1:1 mixture of RPMI1640 medium and Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; GIBCO 3762782S) | Temperature | 37 C |
| Cell Density at Seeding | 1-2 x 10^5 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA |
| Doubling Time | very slow (approx. 2 weeks) | Cell Number in Vial (cells/1ml) | 1.0 x 10^6 |
| Viability at cell freezing (%) | 82 | Antibiotics Used | free |
| Passage Number | PDL | ||
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - basal medium | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |