JCRB2221 A9(Hygro21)

Cell information

Important Notice(s)

ヒト単一染色体含有ライブラリ A9シリーズ細胞株の培養法

Information for the timing of selection for A9-series cell lines

ヒト単染色体導入ハイブリッド細胞の分譲について：鳥取大とのMTA締結が必要です

About the distribution of human monochromosomal hybrid cells: Requirement of MTA between customers and Tottori University

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB2221 Cell Name   A9(Hygro21)
Profile   contains human chromosome #21 (MTA with Tottori Univ. is required.) Other Name   A9
Animal   human chromosome-introduced Strain   C3H
Genus   Mus Species   musclus
Sex    Age   
Identity    Tissue for Primary Cancer   human monochromosomal hybrid panel
Case history    Metastasis   
Tissue Metastasized    Genetics   93.3% cells included intact human chromosome #21 at deposit.
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   skin fibroblast, AG-r. Cells include human chromosome #21.
Classify   panel Established by   Oshimura,M.
Registered by   Oshimura,M. Regulation for Distribution   MTA with Tottori Univ. is required.
Comment   Human chromosome was selected by hygromycine B. Year   1997
Medium   Dulbecco's modified Eagle's medium with 10% calf serum and 1mg/ml Hygromycin B (Sigma). Methods for Passages   Cells are treated with 0.25% trypsin.
Cell Number on Passage   2 x 10^4 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   skin
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2835
Reference
Pubmed id:10919723Human monochromosome hybrid cell panel characterized by FISH in the JCRB/HSRRB.
Tanabe H,Nakagawa Y,Minegishi D,Hashimoto K,Tanaka N,Oshimura M,Sofuni T,Mizusawa H
Chromosome Res. 2000;8(4):319-34
Pubmed id:8851764Cosmids and transcribed sequences from chromosome 11q23.
Katoh M,Nakagawa Y,Yawata T,Kumano S,Kobayashi E,Kurimasa A,Kugoh H,Oshimura M
Jpn J Hum Genet. 1995 Dec;40(4):307-17
Pubmed id:8527393Normal human chromosome 2 induces cellular senescence in the human cervical carcinoma cell line SiHa.
Uejima H,Mitsuya K,Kugoh H,Horikawa I,Oshimura M
Genes Chromosomes Cancer. 1995 Oct;14(2):120-7
Pubmed id:7493906Restoration of the cellular senescence program and repression of telomerase by human chromosome 3.
Ohmura H,Tahara H,Suzuki M,Ide T,Shimizu M,Yoshida MA,Tahara E,Shay JW,Barrett JC,Oshimura M
Jpn J Cancer Res. 1995 Oct;86(10):899-904
Pubmed id:1639397Ordering of human chromosome 3p markers by radiation hybrid mapping.
Tamari M,Hamaguchi M,Shimizu M,Oshimura M,Takayama H,Kohno T,Yamaguchi N,Sugimura T,Terada M,Yokota J
Genomics. 1992 Jul;13(3):705-12
Pubmed id:1723716Human complement regulatory proteins expressed on mouse A9 cells containing a human chromosome 1.
Seya T,Okada M,Hara T,Matsumoto M,Miyagawa S,Oshimura M
Immunology. 1991 Dec;74(4):719-24
Pubmed id:1995301Introduction of new genetic markers on human chromosomes.
Satoh H,Barrett JC,Oshimura M
Exp Cell Res. 1991 Mar;193(1):5-11
Pubmed id:1978855Establishment of a novel immortalized cell line from ataxia telangiectasia fibroblasts and its use for the chromosomal assignment of radiosensitivity gene.
Ejima Y,Oshimura M,Sasaki MS
Int J Radiat Biol. 1990 Dec;58(6):989-97
Pubmed id:2267132Suggestive evidence for functionally distinct, tumor-suppressor genes on chromosomes 1 and 11 for a human fibrosarcoma cell line, HT1080.
Kugoh HM,Hashiba H,Shimizu M,Oshimura M
Oncogene. 1990 Nov;5(11):1637-44
Pubmed id:1702413Normal human chromosome 1 carries suppressor activity for various phenotypes of a Kirsten murine sarcoma virus-transformed NIH/3T3 cell line.
Yamada H,Horikawa I,Hashiba H,Oshimura M
Jpn J Cancer Res. 1990 Nov;81(11):1095-100
Pubmed id:2392323Multiple chromosomes carrying tumor suppressor activity for a uterine endometrial carcinoma cell line identified by microcell-mediated chromosome transfer.
Yamada H,Wake N,Fujimoto S,Barrett JC,Oshimura M
Oncogene. 1990 Aug;5(8):1141-7
Pubmed id:2502516Construction of mouse A9 clones containing a single human chromosome tagged with neomycin-resistance gene via microcell fusion.
Koi M,Shimizu M,Morita H,Yamada H,Oshimura M
Jpn J Cancer Res. 1989 May;80(5):413-8
Pubmed id:2498246Construction of mouse A9 clones containing a single human chromosome (X/autosome translocation) via micro-cell fusion.
Koi M,Morita H,Shimizu M,Oshimura M
Jpn J Cancer Res. 1989 Feb;80(2):122-5
Pubmed id:14213660THREE DEGREES OF GUANYLIC ACID--INOSINIC ACID PYROPHOSPHORYLASE DEFICIENCY IN MOUSE FIBROBLASTS.
LITTLEFIELD JW
Nature. 1964 Sep 12;203():1142-4

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LOT Information

Cell No.   JCRB2221 Cell Name   
LOT No.   04242006 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% calf serum and 1 mg/ml hygromycin B (hygromycin should not be added immediately after thawing but be added at log growth phase. Temperature   37 C
Cell Density at Seeding   4 - 5 x 10^4 cells/ml Methods for Passages   
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   2.6 x 10^6
Viability at cell freezing (%)   95.3 Antibiotics Used   1 mg/ml hygromycin B (Sigma)
Passage Number   P7 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, AST and LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% CS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
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