JCRB2223 A9(BsrX)

Cell information

Important Notice(s)

ヒト単一染色体含有ライブラリ A9シリーズ細胞株の培養法

Information for the timing of selection for A9-series cell lines

ヒト単染色体導入ハイブリッド細胞の分譲について：鳥取大とのMTA締結が必要です

About the distribution of human monochromosomal hybrid cells: Requirement of MTA between customers and Tottori University

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB2223 Cell Name   A9(BsrX)
Profile   contains human chromosome X (MTA with Tottori Univ. is required.) Other Name   A9
Animal   human chromosome-introduced Strain   C3H
Genus   Mus Species   musclus
Sex    Age   
Identity    Tissue for Primary Cancer   human monochromosomal hybrid panel
Case history    Metastasis   
Tissue Metastasized    Genetics   28.0% cells included intact human chromosome X when deposited.
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   skin fibroblast, AG-r. Cells include human chromosome #23.
Classify   panel Established by   Oshimura,M.
Registered by   Oshimura,M. Regulation for Distribution   MTA with Tottori Univ. is required.
Comment   Human chromosome selected by blasticidene S hydrochloride. Tagged site Xp11.2. The human chromosome X was derived from KM primary cells. Year   1997
Medium   Dulbecco's modified Eagle's medium with 10% calf serum and 3umg/ml Blasticidin S hydrochloride (Funakoshi,92-1). Methods for Passages   Cells are treated with 0.25% trypsin.
Cell Number on Passage   2 x 10^4 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   skin
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2832
Reference
Pubmed id:10919723Human monochromosome hybrid cell panel characterized by FISH in the JCRB/HSRRB.
Tanabe H,Nakagawa Y,Minegishi D,Hashimoto K,Tanaka N,Oshimura M,Sofuni T,Mizusawa H
Chromosome Res. 2000;8(4):319-34
Pubmed id:8851764Cosmids and transcribed sequences from chromosome 11q23.
Katoh M,Nakagawa Y,Yawata T,Kumano S,Kobayashi E,Kurimasa A,Kugoh H,Oshimura M
Jpn J Hum Genet. 1995 Dec;40(4):307-17
Pubmed id:8527393Normal human chromosome 2 induces cellular senescence in the human cervical carcinoma cell line SiHa.
Uejima H,Mitsuya K,Kugoh H,Horikawa I,Oshimura M
Genes Chromosomes Cancer. 1995 Oct;14(2):120-7
Pubmed id:7493906Restoration of the cellular senescence program and repression of telomerase by human chromosome 3.
Ohmura H,Tahara H,Suzuki M,Ide T,Shimizu M,Yoshida MA,Tahara E,Shay JW,Barrett JC,Oshimura M
Jpn J Cancer Res. 1995 Oct;86(10):899-904
Pubmed id:1639397Ordering of human chromosome 3p markers by radiation hybrid mapping.
Tamari M,Hamaguchi M,Shimizu M,Oshimura M,Takayama H,Kohno T,Yamaguchi N,Sugimura T,Terada M,Yokota J
Genomics. 1992 Jul;13(3):705-12
Pubmed id:1723716Human complement regulatory proteins expressed on mouse A9 cells containing a human chromosome 1.
Seya T,Okada M,Hara T,Matsumoto M,Miyagawa S,Oshimura M
Immunology. 1991 Dec;74(4):719-24
Pubmed id:1995301Introduction of new genetic markers on human chromosomes.
Satoh H,Barrett JC,Oshimura M
Exp Cell Res. 1991 Mar;193(1):5-11
Pubmed id:1978855Establishment of a novel immortalized cell line from ataxia telangiectasia fibroblasts and its use for the chromosomal assignment of radiosensitivity gene.
Ejima Y,Oshimura M,Sasaki MS
Int J Radiat Biol. 1990 Dec;58(6):989-97
Pubmed id:2267132Suggestive evidence for functionally distinct, tumor-suppressor genes on chromosomes 1 and 11 for a human fibrosarcoma cell line, HT1080.
Kugoh HM,Hashiba H,Shimizu M,Oshimura M
Oncogene. 1990 Nov;5(11):1637-44
Pubmed id:1702413Normal human chromosome 1 carries suppressor activity for various phenotypes of a Kirsten murine sarcoma virus-transformed NIH/3T3 cell line.
Yamada H,Horikawa I,Hashiba H,Oshimura M
Jpn J Cancer Res. 1990 Nov;81(11):1095-100
Pubmed id:2392323Multiple chromosomes carrying tumor suppressor activity for a uterine endometrial carcinoma cell line identified by microcell-mediated chromosome transfer.
Yamada H,Wake N,Fujimoto S,Barrett JC,Oshimura M
Oncogene. 1990 Aug;5(8):1141-7
Pubmed id:2502516Construction of mouse A9 clones containing a single human chromosome tagged with neomycin-resistance gene via microcell fusion.
Koi M,Shimizu M,Morita H,Yamada H,Oshimura M
Jpn J Cancer Res. 1989 May;80(5):413-8
Pubmed id:2498246Construction of mouse A9 clones containing a single human chromosome (X/autosome translocation) via micro-cell fusion.
Koi M,Morita H,Shimizu M,Oshimura M
Jpn J Cancer Res. 1989 Feb;80(2):122-5
Pubmed id:14213660THREE DEGREES OF GUANYLIC ACID--INOSINIC ACID PYROPHOSPHORYLASE DEFICIENCY IN MOUSE FIBROBLASTS.
LITTLEFIELD JW
Nature. 1964 Sep 12;203():1142-4

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LOT Information

Cell No.   JCRB2223 Cell Name   
LOT No.   12252007 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% calf serum and 3ug/ml blasticidin S hydrochloride (do not add blasticidin for a few days from thawing.) Temperature   37 C
Cell Density at Seeding   2 - 5 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   50 hrs Cell Number in Vial (cells/1ml)   approx. 1 x 10^6
Viability at cell freezing (%)   approx. 60 Antibiotics Used   blasticidin was included for selection
Passage Number   P10 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
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