JCRB0648 L.P3
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0648 | Cell Name | L.P3 |
|---|---|---|---|
| Profile | Serum free cell line of the mouse L929 cell line. | Other Name | L.P3 |
| Animal | mouse | Strain | C3H |
| Genus | Mus | Species | musclus |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | skin | |
| Case history | cells were established by Katsuta,H. et.al. by serum free medium. | Metastasis | |
| Tissue Metastasized | Genetics | skin | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epitherial-like | Character | This cell line was surpplied from Riken Genebank for the replacement to the JCRB0605. |
| Classify | transformed | Established by | Katsuta,H. |
| Registered by | Takaoka,T. | Regulation for Distribution | free |
| Comment | Using serum free medium all the time is essential. | Year | 1997 |
| Medium | Although the DM160 medium was indicated but it can be replaced to the DM201 medium. Serum free. Frozen medium FM-1 established by Ohno,T. and destributed from Kyokuto Seiyaku Kogyo.Co. used. | Methods for Passages | Harvest cells by rubber policeman. |
| Cell Number on Passage | 1 x 10^4 cells/sq.cm. | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | connective tissue |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:3242846 | Secretion of a novel cell-adhesive protein distinct from fibronectin by mouse L.P3 cells growing in protein- and lipid-free synthetic medium. Morita H,Yasumitsu H,Harada H,Umeda M,Takaoka T Cell Struct Funct. 1988 Dec;13(6):481-93 |
| Pubmed id:5452761 | Absence of essential fatty acids in mammalian cell strains cultured in lipid-and protein-free chemically defined synthetic media. Kagawa Y,Takaoka T,Katsuta H J Biochem. 1970 Jul;68(1):133-6 |
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LOT Information
| Cell No. | JCRB0648 | Cell Name | L.P3 |
|---|---|---|---|
| LOT No. | 112697 | Lot Specification | distribution |
| Medium | DM160 medium only. No serum added. | Temperature | 37 C |
| Cell Density at Seeding | 1 x 10^4 cells/sq.cm. | Methods for Passages | Cells are harvested with scraper (rubber policeman). |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.1x10^6 |
| Viability at cell freezing (%) | 82.0 | Antibiotics Used | free |
| Passage Number | P4* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | G6PD, NP, LDH examined. Mouse confirmed. |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Frozen medium FM-1 established by Ohno,T. and destributed from Kyokuto Seiyaku Kogyo.Co. used. | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information |
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