JCRB0648 L.P3

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0648 Cell Name   L.P3
Profile   Serum free cell line of the mouse L929 cell line. Other Name   L.P3
Animal   mouse Strain   C3H
Genus   Mus Species   musclus
Sex    Age   
Identity    Tissue for Primary Cancer   skin
Case history   cells were established by Katsuta,H. et.al. by serum free medium. Metastasis   
Tissue Metastasized    Genetics   skin
Life Span   infinite Crisis PDL   
Morphology   epitherial-like Character   This cell line was surpplied from Riken Genebank for the replacement to the JCRB0605.
Classify   transformed Established by   Katsuta,H.
Registered by   Takaoka,T. Regulation for Distribution   free
Comment   Using serum free medium all the time is essential. Year   1997
Medium   Although the DM160 medium was indicated but it can be replaced to the DM201 medium. Serum free. Frozen medium FM-1 established by Ohno,T. and destributed from Kyokuto Seiyaku Kogyo.Co. used. Methods for Passages   Harvest cells by rubber policeman.
Cell Number on Passage   1 x 10^4 cells/sq.cm. Race   
CO2 Conc.   5 % Tissue Sampling   connective tissue
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_U990
Pubmed id:3242846Secretion of a novel cell-adhesive protein distinct from fibronectin by mouse L.P3 cells growing in protein- and lipid-free synthetic medium.
Morita H,Yasumitsu H,Harada H,Umeda M,Takaoka T
Cell Struct Funct. 1988 Dec;13(6):481-93
Pubmed id:5452761Absence of essential fatty acids in mammalian cell strains cultured in lipid-and protein-free chemically defined synthetic media.
Kagawa Y,Takaoka T,Katsuta H
J Biochem. 1970 Jul;68(1):133-6


LOT Information

Cell No.   JCRB0648 Cell Name   L.P3
LOT No.   112697 Lot Specification   distribution
Medium   DM160 medium only. No serum added. Temperature   37 C
Cell Density at Seeding   1 x 10^4 cells/sq.cm. Methods for Passages   Cells are harvested with scraper (rubber policeman).
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.1x10^6
Viability at cell freezing (%)   82.0 Antibiotics Used   free
Passage Number   P4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, NP, LDH examined. Mouse confirmed.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Frozen medium FM-1 established by Ohno,T. and destributed from Kyokuto Seiyaku Kogyo.Co. used. CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information