JCRB0125 L alpha

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0125 Cell Name   L alpha
Profile   L cell line introduced with poliovirus receptor protein alpha gene, thymidine kinase defective Other Name   
Animal   mouse Strain   
Genus   Mus Species   musclus
Sex   F Age   
Identity    Tissue for Primary Cancer   skin
Case history   cells introduced by polio receptor gene.Cells contaminated by Mycoplasma but decontaminated in JCRB. Metastasis   
Tissue Metastasized    Genetics   Mouse L tk- cell line was transformed by cDNA of poliovirus receptor protein alpha.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   This cell line expresses poliovirus receptor and sensitive to the poliovirus.
Classify   transformed Established by   Nomoto,A.
Registered by   Nomoto,A. Regulation for Distribution   free
Comment   Receptors are examined after decontaminaion of mycoplasmas. Year   1997
Medium   Dulbecco's modified Eagle's medium with 5% fetal calf serum. (Mitsubishi PVF01 used as serum)). Methods for Passages   Cells are harvested with 0.125% trypsin and 0.02% EDTA at room temperature for 5 min.
Cell Number on Passage   1.5x10^4 cells/sq.cm. Race   
CO2 Conc.   5 % Tissue Sampling   loose connective tissue
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_U992
Pubmed id:7474095A new cis-acting element for RNA replication within the 5' noncoding region of poliovirus type 1 RNA.
Shiroki K,Ishii T,Aoki T,Kobashi M,Ohka S,Nomoto A
J Virol. 1995 Nov;69(11):6825-32
Pubmed id:8389915Temperature-sensitive mouse cell factors for strand-specific initiation of poliovirus RNA synthesis.
Shiroki K,Kato H,Koike S,Odaka T,Nomoto A
J Virol. 1993 Jul;67(7):3989-96
Pubmed id:1331508A second gene for the African green monkey poliovirus receptor that has no putative N-glycosylation site in the functional N-terminal immunoglobulin-like domain.
Koike S,Ise I,Sato Y,Yonekawa H,Gotoh O,Nomoto A
J Virol. 1992 Dec;66(12):7059-66
Pubmed id:1851992Functional domains of the poliovirus receptor.
Koike S,Ise I,Nomoto A
Proc Natl Acad Sci U S A. 1991 May 15;88(10):4104-8
Pubmed id:2170108The poliovirus receptor protein is produced both as membrane-bound and secreted forms.
Koike S,Horie H,Ise I,Okitsu A,Yoshida M,Iizuka N,Takeuchi K,Takegami T,Nomoto A
EMBO J. 1990 Oct;9(10):3217-24


LOT Information

Cell No.   JCRB0125 Cell Name   L alpha
LOT No.   033098 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 5% fetal calf serum (Mitsubishi, PVF01). Temperature   37 C
Cell Density at Seeding   1-2 x 10^4 cells/sq.cm. Methods for Passages   Cells are harvested with 0.125% trypsin and 0.02% EDATA for 5 min. at room temperature.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.6x10^6
Viability at cell freezing (%)   96.0 Antibiotics Used   free
Passage Number   P15* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information