JCRB0131 RL-33

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0131 Cell Name   RL-33
Profile   Japansese white rabbit lung cell line sensitive to HSV or VSV. Other Name   
Animal   rabbit Strain   Japanese white
Genus    Species   
Sex    Age   Young adult
Identity    Tissue for Primary Cancer   lung
Case history   normal Metastasis   No
Tissue Metastasized    Genetics   HSV,VSV sensitive.Good to study anti-viral agents.
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   Cell growth inhibited by cell-cell contact.
Classify   immortalized Established by   Yoshii,T. & Kono,R.
Registered by   Yoshii,T. Regulation for Distribution   free
Comment    Year   1998
Medium   Eagle's minimal essential medium with 7-8% fetal bovine serum or calf serum and 0.11% NaHCO3. Methods for Passages   Culture at 37 C and harvest cells by treatment with 0.25% trypsin and 0.02% EDTA.
Cell Number on Passage   Split ratio=1/5 (or 8) Race   
CO2 Conc.   5 % Tissue Sampling   lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2709

LOT Information

Cell No.   JCRB0131 Cell Name   RL-33
LOT No.   100698 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% fetal bovine serrum. Temperature   37 C
Cell Density at Seeding   4x10^4 cells/sq.cm. Methods for Passages   Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.4 x 10^6
Viability at cell freezing (%)   90.3 Antibiotics Used   free
Passage Number   P314 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, LD, NP examined. Speceis confirmed.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information