JCRB0132.2 AH601.P3(JTC27)

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0132.2 Cell Name   AH601.P3(JTC27)
Profile   AH-601 cell line adapted to a serum free medium. Other Name   JTC27.P3
Animal   rat Strain   
Genus   Rattus Species   norvegicus
Sex    Age   
Identity    Tissue for Primary Cancer   liver
Case history   tumor transplantable Metastasis   Yes
Tissue Metastasized    Genetics   AH-601 cells adapted to a serum free medium. Serum free cell culture established on 1972. Low passage number.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   transplantable rat ascites tumor P3 cell line
Classify   tumor Established by   Huh,N.,Takaoka,T. & Katsuta,H.
Registered by   Takaoka,T. Regulation for Distribution   free
Comment    Year   1999
Medium   DM201 medium. No serum. Methods for Passages   Cells are harvested with scraper.
Cell Number on Passage   5x10^4 cells/sq.cm. Race   
CO2 Conc.   5 % Tissue Sampling   acites
Tissue Type   hepatoma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_U216
Reference
Pubmed id:4340679Tissue culture of rat ascites hepatoma AH-601 cells.
Huh N,Takaoka T,Katsuta H
Jpn J Exp Med. 1972 Jun;42(3):249-62

Images



LOT Information

Cell No.   JCRB0132.2 Cell Name   AH601.P3(JTC27)
LOT No.   031899 Lot Specification   distribution
Medium   DM201 medium only. No serum used for this cells. Pssage every 7 days. Temperature   37 C
Cell Density at Seeding   2.8 x 10^4 cells/sq.cm. Methods for Passages   Cells harvested with using scraper. Gentle handling required.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   65.1 Antibiotics Used   free but MC210 to remove Mycoplasmas.
Passage Number   P12* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, LDH, and NP examined. (Rat)
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0132.2 Cell Name   AH601.P3(JTC27)
LOT No.   020299 Lot Specification   distribution
Medium   DM201 medium without serum (serum free cell culture required). Temperature   37 C
Cell Density at Seeding   5x10^4 cells/sq.cm. Methods for Passages   Cells are harvested with scraper.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.1 x 10^6
Viability at cell freezing (%)   77.4 Antibiotics Used   MC210 to decontaminate mycoplasmas.
Passage Number   P10* PDL   NT
Sterility: MYCOPLASMA   +- Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   FM-1 medium with 10% DMSO. CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
CLOSE