JCRB0133 KHM-1B

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0133 Cell Name   KHM-1B
Profile   myeloma, multiple, with hyperamylasemia. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   53
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   IgA lambda multiple myeloma with hyperamylasemia. Metastasis   No
Tissue Metastasized    Genetics   HLA-DR,PCA-1 were positive.
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   Amylase and IgA productions were positively elevated.
Classify   transformed Established by   Matsuzaki,H. and Takatsuki,K.
Registered by   Matsuzaki,H. Regulation for Distribution   
Comment    Year   1999
Medium   RPMI1640 medium with 20 % fetal calf serum was used at the begining. The FCS concentration was reduced to 10% when the cells began to grow steadily. Methods for Passages   
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   pleural effusion
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2972
Reference
Pubmed id:1701504Genetic analysis of amylase-producing cell lines: ectopic activation of the amylase gene by translocation.
Hata H,Matsuzaki H,Sanada I,Takatsuki K
Jpn J Clin Oncol. 1990 Sep;20(3):246-51
Pubmed id:2109452Autocrine growth by two cytokines, interleukin-6 and tumor necrosis factor alpha, in the myeloma cell line KHM-1A.
Hata H,Matsuzaki H,Takatsuki K
Acta Haematol. 1990;83(3):133-6
Pubmed id:2458822Ectopic production of salivary-type amylase by a IgA-lambda-type multiple myeloma.
Hata H,Matsuzaki H,Tanaka K,Nomura H,Kagimoto T,Takeya M,Yamane N,Takatsuki K
Cancer. 1988 Oct 15;62(8):1511-5
Pubmed id:2458153Establishment and characterization of an amylase-producing human myeloma cell line.
Matsuzaki H,Hata H,Takeya M,Takatsuki K
Blood. 1988 Sep;72(3):978-82

Images



LOT Information

Cell No.   JCRB0133 Cell Name   
LOT No.   09172004 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum. Use 20% fetal bovine serum up to log-growth phese from thawing. Temperature   37 C
Cell Density at Seeding   1 - 2 x 10^5 cells/ml Methods for Passages   Dilution (simple addition of medium up to log-growth phese)
Doubling Time   69 hrs Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   90 Antibiotics Used   free
Passage Number   >P16 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmes as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0133 Cell Name   KHM-1B
LOT No.   020599 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal calf serum (Summit 8.1E41). Temperature   37 C
Cell Density at Seeding   1-2x10^6 cells/ml Methods for Passages   Simple dilution (suspension culture).
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.3 x 10^6
Viability at cell freezing (%)   74.2 Antibiotics Used   free
Passage Number   P10* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, LDH, and NP examined (human).
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0133 Cell Name   KHM-1B
LOT No.   07152015 Lot Specification   distribution
Medium   RPMI1640 medium with 20 % fetal bovine serum was used at the begining of culture. The FBS concentration was reduced to 10% when the cells began to grow steadily. Temperature   37 C
Cell Density at Seeding   2.2 - 3.4 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture).
Doubling Time   87 - 98 hrs Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   75.0 Antibiotics Used   free
Passage Number   P14 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11
D13S317:11
D7S820:10
D16S539:9
VWA:14,17
TH01:6
AM:X
TPOX:11
CSF1PO:10
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   75.0 Additional information   
Cell No.   JCRB0133 Cell Name   KHM-1B
LOT No.   02152019 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396). At beginning of culture,RPMI1640 medium(GIBCO) with 20% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   1-5x10^5 cells/ml Methods for Passages   dilution(1/2-1/4)
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.83x10^6
Viability at cell freezing (%)   87.2 Antibiotics Used   free
Passage Number   p14* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11
D13S317:11
D7S820:10
D16S539:9
VWA:14,17
TH01:6
AM:X
TPOX:11
CSF1PO:10
Adhesion   No Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   1.44x10^6/ampou Additional information   At beginning of culture,RPMI1640 medium(GIBCO) with 20% heat inactivated fetal bovine serum(SIGMA12J396). After that,RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396)
Images
Cell No.   JCRB0133 Cell Name   KHM-1B
LOT No.   07202021 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Biowest Cat. # S1820-500) Temperature   37 C
Cell Density at Seeding   0.5 - 2.0 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture). The growth immediately after thawing is considerably slow (doubling time during first one week is 7 days)
Doubling Time   Slow (74 hrs at log phase) Cell Number in Vial (cells/1ml)   3.4 x 10^6
Viability at cell freezing (%)   89 Antibiotics Used   free
Passage Number   Unknown (13 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   82 Additional information   
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