JCRB0136.1 CHO(pMAM-luc)

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0136.1 Cell Name   CHO(pMAM-luc)
Profile   pMAM-luc transfected CHO-K1, luciferase activated by dexamethasone Other Name   
Animal   hamster, Chinese Strain   Chinese
Genus   Cricetulus Species   griseus
Sex    Age   
Identity    Tissue for Primary Cancer   ovary
Case history   genetically engineered CHO-K1 cells. External gene fragment(s) are introduced. Metastasis   
Tissue Metastasized    Genetics   Plasmid pMAM-luc transfected CHO-K1 cells. Luciferase activated by dexamethasone(2uM) at 37C, 5 hrs.
Life Span   infinite Crisis PDL   
Morphology    Character   Luciferase gene under control of MMTV-LTR in plasmid.
Classify   transformed Established by   Mizuno,S. et al.
Registered by   Mizuno,S. Regulation for Distribution   not identified
Comment   See CHO-K1 cell culture methods. Year   1998
Medium   Ham's F12 medium with 10% fetal bovine serum. Methods for Passages   Cells are harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Cell Number on Passage   split ratio=1/10 Race   
CO2 Conc.   5 % Tissue Sampling   ovary
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Reference
Pubmed id:9538196Suppression of thermotolerance development through cycloheximide-induced negative control of stress protein gene expression.
Akagawa H,Ishii A,Mizuno S
J Biochem. 1998 Feb;123(2):226-32
Pubmed id:9113384Stress dose-dependent suppression of heat shock protein gene expression by inhibiting protein synthesis during heat shock treatment.
Mizuno S,Ishii A,Murakami Y,Akagawa H
Cell Struct Funct. 1997 Feb;22(1):7-13
Pubmed id:8689565Heat shock transcription factors: structure and regulation.
Wu C
Annu Rev Cell Dev Biol. 1995;11():441-69
Pubmed id:8355691The DNA-binding activity of the human heat shock transcription factor is regulated in vivo by hsp70.
Mosser DD,Duchaine J,Massie B
Mol Cell Biol. 1993 Sep;13(9):5427-38
Pubmed id:3422754Expression of the firefly luciferase gene in vaccinia virus: a highly sensitive gene marker to follow virus dissemination in tissues of infected animals.
Rodriguez JF,Rodriguez D,Rodriguez JR,McGowan EB,Esteban M
Proc Natl Acad Sci U S A. 1988 Mar;85(5):1667-71
Pubmed id:3821727Firefly luciferase gene: structure and expression in mammalian cells.
de Wet JR,Wood KV,DeLuca M,Helinski DR,Subramani S
Mol Cell Biol. 1987 Feb;7(2):725-37
Pubmed id:17758108Transient and stable expression of the firefly luciferase gene in plant cells and transgenic plants.
Ow DW,DE Wet JR,Helinski DR,Howell SH,Wood KV,Deluca M
Science. 1986 Nov 14;234(4778):856-9
Pubmed id:3927293Isolation and functional analysis of a human 70,000-dalton heat shock protein gene segment.
Voellmy R,Ahmed A,Schiller P,Bromley P,Rungger D
Proc Natl Acad Sci U S A. 1985 Aug;82(15):4949-53
Pubmed id:5271752Genetics of somatic mammalian cells. X. Complementation analysis of glycine-requiring mutants.
Kao F,Chasin L,Puck TT
Proc Natl Acad Sci U S A. 1969 Dec;64(4):1284-91

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LOT Information

Cell No.   JCRB0136.1 Cell Name   
LOT No.   092999 Lot Specification   distribution
Medium   Ham's F12 medium with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   1-2 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   D.T.=ca 24 hrs. Cell Number in Vial (cells/1ml)   2.2 x 10^6
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   P8* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as Chinese hamster by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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