JCRB0536 TIG-121
Cell information
Important Notice(s)Notes for the cultivation of human normal diploid cells (JCRB05**) : ヒト正常二倍体細胞の取り扱い上の注意点
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0536 | Cell Name | TIG-121 |
|---|---|---|---|
| Profile | skin, normal diploid fibroblast | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 8-month-old |
| Identity | available | Tissue for Primary Cancer | skin (leg) |
| Case history | primary, normal fibroblast | Metastasis | |
| Tissue Metastasized | Genetics | ||
| Life Span | finite | Crisis PDL | |
| Morphology | fibroblast-like | Character | normal diploid |
| Classify | Established by | ||
| Registered by | Tokyo Metropolitan I | Regulation for Distribution | |
| Comment | In the paper referring this cell line (Exp. Cell Res. 220: 501 (1995)), the cell name was mistyped as TIG-120 (in Material & Method section). | Year | 1999 |
| Medium | Eagle's minimum essential medium with 10% fetal bovine serum | Methods for Passages | trypsin-EDTA |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:7556460 | Fetal-adult phenotype transition, in terms of the serum dependency and growth factor requirements, of human skin fibroblast migration. Kondo H,Yonezawa Y Exp Cell Res. 1995 Oct;220(2):501-4 |
LOT Information
| Cell No. | JCRB0536 | Cell Name | |
|---|---|---|---|
| LOT No. | 09272010 | Lot Specification | distribution |
| Medium | Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat. # 10099). This lot was frozen using DMSO. | Temperature | 37 C |
| Cell Density at Seeding | approx. 1.5 x 10^4 cells/ml | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 42 hrs. | Cell Number in Vial (cells/1ml) | 4.6 x 10^5 |
| Viability at cell freezing (%) | 92.7 | Antibiotics Used | free |
| Passage Number | Unknown (3 at bank) | PDL | 18 |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - EMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0536 | Cell Name | |
|---|---|---|---|
| LOT No. | R211 | Lot Specification | distribution |
| Medium | Eagle's minimal essential medium with 10% FBS This lot of ampule was frozen using DMSO. | Temperature | 37 C |
| Cell Density at Seeding | split ratio = 1:4 | Methods for Passages | 0.25% trypsin and 0.02% EDTA |
| Doubling Time | Cell Number in Vial (cells/1ml) | 1.6 x 10^6 | |
| Viability at cell freezing (%) | 90.7 | Antibiotics Used | 100 units/ml penicillin and 100 ug/ml streptomysin |
| Passage Number | PDL | 18 | |
| Sterility: MYCOPLASMA | Sterility: BACTERIA | - | |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |