JCRB0139 TASK1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0139 Cell Name   TASK1
Profile   Human cell line with primitive neuroectodermal tumor (Askin's tumor). Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   21
Identity   available Tissue for Primary Cancer   neural
Case history   neuroectodermal tumor. Metastasis   No
Tissue Metastasized    Genetics   NSE,vimentin, & MIC2 positive, NF & N-CAM negative
Life Span   infinite Crisis PDL   
Morphology   create aggregates Character   Primitive neuroectodermal tumor (Askin's tumor).
Classify   tumor Established by   Horiguchi,H. et al.
Registered by   Matsui,M. Regulation for Distribution   not defined.
Comment   Cells are originally contaminated by mycoplasmas and decontaminated by the MC210 in the JCRB cell bank. Year   1999
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum. Methods for Passages   Cells are harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Cell Number on Passage   1.5 x 10^4 cells/sq.cm Race   Japanese
CO2 Conc.   5 % Tissue Sampling   chest wall mass/left
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3177
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 1299067
Reference
Pubmed id:9661044Establishment and characterization of a typical primitive neuroectodermal tumor cell line.
Horiguchi H,Matsui M,Ohtsubo R,Ogata T,Kaneko M,Fujiwara M,Kamma H
In Vitro Cell Dev Biol Anim. 1998 Jun;34(6):439-42

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LOT Information

Cell No.   JCRB0139 Cell Name   TASK1
LOT No.   07032000 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum (Intergen RB2405). Temperature   37 C
Cell Density at Seeding   1.0 x 10^4 cells/sq.cm. Methods for Passages   Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA. Change medium at one day after starting culture then every 3-4 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.5 x 10^6
Viability at cell freezing (%)   92.9 Antibiotics Used   free
Passage Number   P12* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, LDH, G6PD (typeA). Human.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:13
D13S317:8
D7S820:12
D16S539:12
VWA:14,18
TH01:9
AM:X
TPOX:11
CSF1PO:10
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium containing 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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