JCRB0143.4 F2408 EBNA2 cl-24
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0143.4 | Cell Name | F2408 EBNA2 cl-24 |
|---|---|---|---|
| Profile | EBNA2-transfected, (Fischer rat) | Other Name | F2408 EBNA2 |
| Animal | rat | Strain | Fischer |
| Genus | Rattus | Species | norvegicus |
| Sex | Age | embryo | |
| Identity | Tissue for Primary Cancer | embryo | |
| Case history | recombinant plasmid pZE2 with coding region of the EBNA2 was transfected | Metastasis | |
| Tissue Metastasized | Genetics | Expression of the EBNA2 was strong. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | fibroblast-like | Character | fibroblast-like |
| Classify | transformed | Established by | W. Eckhalt |
| Registered by | Shimakage,M. | Regulation for Distribution | free |
| Comment | Cell culture becomes difficult when inoculum size is lower than 1x10^4 cells/cm^2. Be care about overgrowth when inoculum size is over 3 x 10^4 cells/cm^2. | Year | 1999 |
| Medium | Dulbecco's modified Eagle's medium with 5% fetal calf serum. | Methods for Passages | Cells are treated with 0.25% trypsin and 0.02% EDTA when subculture cells and subculture cells every 4 days. |
| Cell Number on Passage | 2 x 10^5 cells/6 cm dish. | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | whole body of embryo |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:8938143 | Increased sensitivity of EBNA2-transformed rat fibroblasts to ionizing radiation. Shimakage M,Miyata Y,Inoue H,Yutsudo M,Hakura A Int J Cancer. 1996 Nov 27;68(5):612-5 |
| Pubmed id:7556601 | Tumorigenicity of EBNA2-transfected cells. Shimakage M,Kurata A,Inoue H,Okamoto Y,Yutsudo M,Hakura A FEBS Lett. 1995 Sep 11;371(3):245-8 |
| Pubmed id:6307528 | Cellular responsiveness to growth factors correlates with a cell's ability to express the transformed phenotype. Kaplan PL,Ozanne B Cell. 1983 Jul;33(3):931-8 |
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LOT Information
| Cell No. | JCRB0143.4 | Cell Name | F2408 EBNA2 cl-24 |
|---|---|---|---|
| LOT No. | 101999 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 5% fetal calf serum (Mitsubishi PFCS PVF001) | Temperature | 37 C |
| Cell Density at Seeding | 2.2 x 10^4 cells/sq.cm. | Methods for Passages | Cells harvested after treatment with 0.25% trypsin-0.02% EDTA treatment and dilute. Subculture every 5-7 days. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.9 x 10^6 |
| Viability at cell freezing (%) | 97.9 | Antibiotics Used | free |
| Passage Number | P5* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | G6PD, NP and LD are examined. Rat confirmed. |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Culture medium with 5% DMSO. | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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