JCRB0146 MLMA

Cell information

Important Notice(s)

Note on the adhesion of JCRB0146 MLMA to culture substratum / JCRB0146 MLMAの接着性

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0146 Cell Name   MLMA
Profile   The cells are IgD, IgM positive, hairy B-cells from human malignant lymphoma patient. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   32
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   malignant lymphoma Metastasis   No
Tissue Metastasized    Genetics   47 X,-X (X,0)
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   IgD, IgM positive, hairy B-cell line
Classify   tumor Established by   Matsuda,K.
Registered by   Matsuda,K. Regulation for Distribution   No regulation.
Comment    Year   2000
Medium    Methods for Passages   
Cell Number on Passage    Race   Japanese
CO2 Conc.   5 % Tissue Sampling   peritoneal effusion
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1419
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 908142
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LOT Information

Cell No.   JCRB0146 Cell Name   MLMA
LOT No.   03032000 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal calf serum (Intergen RB2405). Temperature   37 C
Cell Density at Seeding   1.5 x 10^5 cells/ml Methods for Passages   Simple dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.4 x 10^6
Viability at cell freezing (%)   97.0 Antibiotics Used   free
Passage Number   P12* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeA), LDH, NP. Human.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
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Cell No.   JCRB0146 Cell Name   MLMA
LOT No.   08272014 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   0.5 - 1.8 x 10^5 cells/mL Methods for Passages   Pipetting. This cell line is reported as adherent culture, but revealed to attach to cell culture flask for suspension culture.
Doubling Time   D.T. = approx. 62 hrs Cell Number in Vial (cells/1ml)   8.0 x 10^5
Viability at cell freezing (%)   89.5 Antibiotics Used   free
Passage Number   Unknown (14 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11
D13S317:8,10
D7S820:10
D16S539:9,12
VWA:16,17
TH01:6,7
AM:X
TPOX:8
CSF1PO:12,14
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   89.5
Additional information   
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