JCRB0148 KHM-5M
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0148 | Cell Name | KHM-5M |
|---|---|---|---|
| Profile | Undifferentiated human thyroid cell line producing interl eukin 8. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 65 |
| Identity | available | Tissue for Primary Cancer | thyroid |
| Case history | hard tumors in the anterior right cervical and supraclavicular region. One of them involved thyroid. | Metastasis | |
| Tissue Metastasized | Genetics | Cells are derived from thyroid. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | fibroblast-like | Character | Neutrophil chemotactic factor (inter leukin 8) producing cell line. |
| Classify | tumor | Established by | Matsuzaki,H. & Takatsuki,K. |
| Registered by | Matsuzaki,H. | Regulation for Distribution | |
| Comment | Cells are deconaminated in the JCRB cellbank. | Year | 1997 |
| Medium | RPMI1640 medium with 20% fetal calf serum at begining of the culture. Serum concentration was reduced to 10 % when cells started to grow steadily . | Methods for Passages | 0.25% trypsin and 0.02% EDTA in CA, MG-free PBS. |
| Cell Number on Passage | 5x10^6 cells/ml | Race | Japanese |
| CO2 Conc. | 5 % | Tissue Sampling | pleural effusion |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:1728417 | Neutrophil chemotactic factors produced by a cell line from thyroid carcinoma. Yoshida M,Matsuzaki H,Sakata K,Takeya M,Kato K,Mizushima S,Kawakita M,Takatsuki K Cancer Res. 1992 Jan 15;52(2):464-9 |
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LOT Information
| Cell No. | JCRB0148 | Cell Name | |
|---|---|---|---|
| LOT No. | 09272002 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% fetal bovine serum (FBS lot; BioWhittaker 0S069F) | Temperature | 37 C |
| Cell Density at Seeding | 5 x 10^4 | Methods for Passages | 0.25% trypsin and 0.02% EDTA in CA, MG-free PBS |
| Doubling Time | 27 hrs | Cell Number in Vial (cells/1ml) | 1.6 x 10^6 |
| Viability at cell freezing (%) | 88.8 | Antibiotics Used | free |
| Passage Number | Unknown (17 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), LD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS-RPMI1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |